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Cloning of mice to six generations

Author

Listed:
  • Teruhiko Wakayama

    (John A. Burns School of Medicine, University of Hawaii
    The Rockefeller University)

  • Yoichi Shinkai

    (Institute for Virus Research, Kyoto University)

  • Kellie L. K. Tamashiro

    (John A. Burns School of Medicine, University of Hawaii)

  • Hiroyuki Niida

    (Lawrence Berkeley National Laboratory, University of California)

  • D. Caroline Blanchard

    (Bekesy Laboratory of Neurobiology, University of Hawaii)

  • Robert J. Blanchard

    (Bekesy Laboratory of Neurobiology, University of Hawaii)

  • Atsuo Ogura

    (National Institute of Infectious Disease)

  • Kentaro Tanemura

    (National Institute of Infectious Disease)

  • Makoto Tachibana

    (Institute for Virus Research, Kyoto University)

  • Anthony C. F. Perry

    (The Rockefeller University)

  • Diana F. Colgan

    (The Rockefeller University)

  • Peter Mombaerts

    (The Rockefeller University)

  • Ryuzo Yanagimachi

    (John A. Burns School of Medicine, University of Hawaii)

Abstract

Mice have been cloned by nuclear transfer into enucleated oocytes1,2,3, and here we describe the reiterative cloning of mice to four and six generations in two independent lines. Successive generations showed no signs of prematureageing, as judged by gross behaviouralparameters, and there was no evidence of shortening of telomeres at the ends of chromosomes, normally an indicator of cellular senescence — in fact, these appeared to increase slightly in length. This increase is surprising, given that the number of mitotic divisions greatly exceeds that of sexually produced animals and that any deleterious effects of cloning might be expected to be amplified in sequentially cloned mice. Our results offer a new approach to the study of organismal ageing.

Suggested Citation

  • Teruhiko Wakayama & Yoichi Shinkai & Kellie L. K. Tamashiro & Hiroyuki Niida & D. Caroline Blanchard & Robert J. Blanchard & Atsuo Ogura & Kentaro Tanemura & Makoto Tachibana & Anthony C. F. Perry & D, 2000. "Cloning of mice to six generations," Nature, Nature, vol. 407(6802), pages 318-319, September.
    • Handle: RePEc:nat:nature:v:407:y:2000:i:6802:d:10.1038_35030301
    DOI: 10.1038/35030301
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