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The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans

Author

Listed:
  • Zhaolan Zhou

    (Harvard Medical School)

  • Ming-juan Luo

    (Harvard Medical School)

  • Katja Straesser

    (University of Heidelberg, Biochemie-Zentrum Heidelberg (BZH))

  • Jun Katahira

    (University of Heidelberg, Biochemie-Zentrum Heidelberg (BZH))

  • Ed Hurt

    (University of Heidelberg, Biochemie-Zentrum Heidelberg (BZH))

  • Robin Reed

    (Harvard Medical School)

Abstract

In metazoans, most pre-messenger RNAs contain introns that are removed by splicing. The spliced mRNAs are then exported to the cytoplasm. Recent studies showed that splicing promotes efficient mRNA export1, but the mechanism for coupling these two processes is not known. Here we show that Aly, the metazoan homologue of the yeast mRNA export factor Yra1p (ref. 2), is recruited to messenger ribonucleoprotein (mRNP) complexes generated by splicing. In contrast, Aly does not associate with mRNPs assembled on identical mRNAs that already have no introns or with heterogenous nuclear RNP (hnRNP) complexes. Aly is recruited during spliceosome assembly, and then becomes tightly associated with the spliced mRNP. Aly shuttles between the nucleus and cytoplasm, and excess recombinant Aly increases both the rate and efficiency of mRNA export in vivo. Consistent with its splicing-dependent recruitment, Aly co-localizes with splicing factors in the nucleus. We conclude that splicing is required for efficient mRNA export as a result of coupling between the splicing and the mRNA export machineries.

Suggested Citation

  • Zhaolan Zhou & Ming-juan Luo & Katja Straesser & Jun Katahira & Ed Hurt & Robin Reed, 2000. "The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans," Nature, Nature, vol. 407(6802), pages 401-405, September.
    • Handle: RePEc:nat:nature:v:407:y:2000:i:6802:d:10.1038_35030160
    DOI: 10.1038/35030160
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    Cited by:

    1. Ross A. Cordiner & Yuhui Dou & Rune Thomsen & Andrii Bugai & Sander Granneman & Torben Heick Jensen, 2023. "Temporal-iCLIP captures co-transcriptional RNA-protein interactions," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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