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Production of gene-targeted sheep by nuclear transfer from cultured somatic cells

Author

Listed:
  • K. J. McCreath

    (PPL Therapeutics Ltd, Roslin)

  • J. Howcroft

    (PPL Therapeutics Ltd, Roslin)

  • K. H. S. Campbell

    (PPL Therapeutics Ltd, Roslin
    University of Nottingham, School of Biological Sciences)

  • A. Colman

    (PPL Therapeutics Ltd, Roslin)

  • A. E. Schnieke

    (PPL Therapeutics Ltd, Roslin)

  • A.J. Kind

    (PPL Therapeutics Ltd, Roslin)

Abstract

It is over a decade since the first demonstration that mouse embryonic stem cells could be used to transfer a predetermined genetic modification to a whole animal1. The extension of this technique to other mammalian species, particularly livestock, might bring numerous biomedical benefits, for example, ablation of xenoreactive transplantation antigens, inactivation of genes responsible for neuropathogenic disease and precise placement of transgenes designed to produce proteins for human therapy. Gene targeting has not yet been achieved in mammals other than mice, however, because functional embryonic stem cells have not been derived. Nuclear transfer from cultured somatic cells provides an alternative means of cell-mediated transgenesis2,3. Here we describe efficient and reproducible gene targeting in fetal fibroblasts to place a therapeutic transgene at the ovine α1(I) procollagen (COL1A1) locus and the production of live sheep by nuclear transfer.

Suggested Citation

  • K. J. McCreath & J. Howcroft & K. H. S. Campbell & A. Colman & A. E. Schnieke & A.J. Kind, 2000. "Production of gene-targeted sheep by nuclear transfer from cultured somatic cells," Nature, Nature, vol. 405(6790), pages 1066-1069, June.
  • Handle: RePEc:nat:nature:v:405:y:2000:i:6790:d:10.1038_35016604
    DOI: 10.1038/35016604
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    Cited by:

    1. M. Samiec & M. Skrzyszowska, 2005. "Microsurgical nuclear transfer by intraooplasmic karyoplast injection as an alternative embryo reconstruction method in somatic cloning of pigs and other mammal species; application value of the metho," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 50(6), pages 235-242.

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