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An electroneutral sodium/bicarbonate cotransporter NBCn1 and associated sodium channel

Author

Listed:
  • Inyeong Choi

    (Yale University School of Medicine)

  • Christian Aalkjaer

    (University of Aarhus)

  • Emile L. Boulpaep

    (Yale University School of Medicine)

  • Walter F. Boron

    (Yale University School of Medicine)

Abstract

Two electroneutral, Na+-driven HCO-3 transporters, the Na+-driven Cl-/HCO-3 exchanger and the electroneutral Na+/ HCO-3 cotransporter, have crucial roles in regulating intracellular pH in a variety of cells, including cardiac myocytes1,2, vascular smooth-muscle3,4, neurons5 and fibroblasts6; however, it is difficult to distinguish their Cl- dependence in mammalian cells. Here we report the cloning of three variants of an electroneutral Na+/HCO-3 cotransporter, NBCn1, from rat smooth muscle. They are 89–92% identical to a human skeletal muscle clone7, 55–57% identical to the electrogenic NBCs and 33–43% identical to the anion exchangers8. When expressed in Xenopus oocytes, NBCn1-B (which encodes 1,218 amino acids) is electroneutral, Na+-dependent and HCO-3-dependent, but not Cl--dependent. Oocytes injected with low levels of NBCn1-B complementary RNA exhibit a Na+ conductance that 4,4-diisothiocyanatostilbene-2,2′-disulphonate stimulates slowly and irreversibly.

Suggested Citation

  • Inyeong Choi & Christian Aalkjaer & Emile L. Boulpaep & Walter F. Boron, 2000. "An electroneutral sodium/bicarbonate cotransporter NBCn1 and associated sodium channel," Nature, Nature, vol. 405(6786), pages 571-575, June.
    • Handle: RePEc:nat:nature:v:405:y:2000:i:6786:d:10.1038_35014615
    DOI: 10.1038/35014615
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