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Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1

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  • Maio S. Chen

    (Brain Research Institute, University of Zurich and Swiss Federal Institute of Technology Zurich)

  • Andrea B. Huber

    (Brain Research Institute, University of Zurich and Swiss Federal Institute of Technology Zurich)

  • Marjan E. van der Haar

    (Brain Research Institute, University of Zurich and Swiss Federal Institute of Technology Zurich)

  • Marcus Frank

    (Brain Research Institute, University of Zurich and Swiss Federal Institute of Technology Zurich)

  • Lisa Schnell

    (Brain Research Institute, University of Zurich and Swiss Federal Institute of Technology Zurich)

  • Adrian A. Spillmann

    (Brain Research Institute, University of Zurich and Swiss Federal Institute of Technology Zurich)

  • Franziska Christ

    (Brain Research Institute, University of Zurich and Swiss Federal Institute of Technology Zurich)

  • Martin E. Schwab

    (Brain Research Institute, University of Zurich and Swiss Federal Institute of Technology Zurich)

Abstract

The capacity of the adult brain and spinal cord to repair lesions by axonal regeneration or compensatory fibre growth is extremely limited. A monoclonal antibody (IN-1) raised against NI-220/250, a myelin protein that is a potent inhibitor of neurite growth, promoted axonal regeneration and compensatory plasticity following lesions of the central nervous system (CNS) in adult rats1,2,3,4. Here we report the cloning of nogo A, the rat complementary DNA encoding NI-220/250. The nogo gene encodes at least three major protein products (Nogo-A, -B and -C). Recombinant Nogo-A is recognized by monoclonal antibody IN-1, and it inhibits neurite outgrowth from dorsal root ganglia and spreading of 3T3 fibroblasts in an IN-1-sensitive manner. Antibodies against Nogo-A stain CNS myelin and oligodendrocytes and allow dorsal root ganglion neurites to grow on CNS myelin and into optic nerve explants. These data show that Nogo-A is a potent inhibitor of neurite growth and an IN-1 antigen produced by oligodendrocytes, and may allow the generation of new reagents to enhance CNS regeneration and plasticity.

Suggested Citation

  • Maio S. Chen & Andrea B. Huber & Marjan E. van der Haar & Marcus Frank & Lisa Schnell & Adrian A. Spillmann & Franziska Christ & Martin E. Schwab, 2000. "Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1," Nature, Nature, vol. 403(6768), pages 434-439, January.
    • Handle: RePEc:nat:nature:v:403:y:2000:i:6768:d:10.1038_35000219
    DOI: 10.1038/35000219
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    Cited by:

    1. Anthony G. Boghdadi & Joshua Spurrier & Leon Teo & Mingfeng Li & Mario Skarica & Benjamin Cao & William C. Kwan & Tobias D. Merson & Susan K. Nilsson & Nenad Sestan & Stephen M. Strittmatter & James A, 2021. "NogoA-expressing astrocytes limit peripheral macrophage infiltration after ischemic brain injury in primates," Nature Communications, Nature, vol. 12(1), pages 1-16, December.

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