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The joining of ribosomal subunits in eukaryotes requires eIF5B

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Listed:
  • Tatyana V. Pestova

    (Department of Microbiology and Immunology State University of New York Health Science Center at Brooklyn
    A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University)

  • Ivan B. Lomakin

    (Department of Microbiology and Immunology State University of New York Health Science Center at Brooklyn)

  • Joon H. Lee

    (Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, National Institutes of Health)

  • Sang Ki Choi

    (Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, National Institutes of Health)

  • Thomas E. Dever

    (Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, National Institutes of Health)

  • Christopher U. T. Hellen

    (Department of Microbiology and Immunology State University of New York Health Science Center at Brooklyn)

Abstract

Initiation of eukaryotic protein synthesis begins with the ribosome separated into its 40S and 60S subunits1. The 40S subunit first binds eukaryotic initiation factor (eIF) 3 and an eIF2–GTP–initiator transfer RNA ternary complex. The resulting complex requires eIF1, eIF1A, eIF4A, eIF4B and eIF4F to bind to a messenger RNA and to scan to the initiation codon2. eIF5 stimulates hydrolysis of eIF2-bound GTP and eIF2 is released from the 48S complex formed at the initiation codon before it is joined by a 60S subunit to form an active 80S ribosome3,4,5,6,7,8. Here we show that hydrolysis of eIF2-bound GTP induced by eIF5 in 48S complexes is necessary but not sufficient for the subunits to join. A second factor termed eIF5B (relative molecular mass 175,000) is essential for this process. It is a homologue of the prokaryotic initiation factor IF2 (refs 6, 7) and, like it8,9,10,11,12, mediates joining of subunits and has a ribosome-dependent GTPase activity that is essential for its function.

Suggested Citation

  • Tatyana V. Pestova & Ivan B. Lomakin & Joon H. Lee & Sang Ki Choi & Thomas E. Dever & Christopher U. T. Hellen, 2000. "The joining of ribosomal subunits in eukaryotes requires eIF5B," Nature, Nature, vol. 403(6767), pages 332-335, January.
    • Handle: RePEc:nat:nature:v:403:y:2000:i:6767:d:10.1038_35002118
    DOI: 10.1038/35002118
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