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The RCAF complex mediates chromatin assembly during DNA replication and repair

Author

Listed:
  • Jessica K. Tyler

    (University of California at San Diego)

  • Christopher R. Adams

    (National Institutes of Health, NICHD)

  • Shaw-Ree Chen

    (University of California at San Diego
    University of Washington)

  • Ryuji Kobayashi

    (Cold Spring Harbor Laboratory)

  • Rohinton T. Kamakaka

    (Cold Spring Harbor Laboratory)

  • James T. Kadonaga

    (University of California at San Diego)

Abstract

Chromatin assembly is a fundamental biological process that is essential for the replication and maintenance of the eukaryotic genome1,2,3,4. In dividing cells, newly synthesized DNA is rapidly assembled into chromatin by the deposition of a tetramer of the histone proteins H3 and H4, followed by the deposition of two dimers of histones H2A and H2B to complete the nucleosome—the fundamental repeating unit of chromatin5. Here we describe the identification, purification, cloning, and characterization of replication-coupling assembly factor (RCAF), a novel protein complex that facilitates the assembly of nucleosomes onto newly replicated DNA in vitro. RCAF comprises the Drosophila homologue of anti-silencing function 1 protein ASF16 and histones H3 and H4. The specific acetylation pattern of H3 and H4 in RCAF is identical to that of newly synthesized histones. Genetic analyses in Saccharomyces cerevisiae demonstrate that ASF1 is essential for normal cell cycle progression, and suggest that RCAF mediates chromatin assembly after DNA replication and the repair of double-strand DNA damage in vivo.

Suggested Citation

  • Jessica K. Tyler & Christopher R. Adams & Shaw-Ree Chen & Ryuji Kobayashi & Rohinton T. Kamakaka & James T. Kadonaga, 1999. "The RCAF complex mediates chromatin assembly during DNA replication and repair," Nature, Nature, vol. 402(6761), pages 555-560, December.
    • Handle: RePEc:nat:nature:v:402:y:1999:i:6761:d:10.1038_990147
    DOI: 10.1038/990147
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