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CtBP/BARS induces fission of Golgi membranes by acylating lysophosphatidic acid

Author

Listed:
  • Roberto Weigert

    (Istituto di Ricerche Farmacologiche “Mario Negri”, Consorzio Mario Negri Sud)

  • Maria Giuseppina Silletta

    (Istituto di Ricerche Farmacologiche “Mario Negri”, Consorzio Mario Negri Sud)

  • Stefania Spanò

    (Istituto di Ricerche Farmacologiche “Mario Negri”, Consorzio Mario Negri Sud)

  • Gabriele Turacchio

    (Istituto di Ricerche Farmacologiche “Mario Negri”, Consorzio Mario Negri Sud)

  • Claudia Cericola

    (Istituto di Ricerche Farmacologiche “Mario Negri”, Consorzio Mario Negri Sud)

  • Antonino Colanzi

    (Istituto di Ricerche Farmacologiche “Mario Negri”, Consorzio Mario Negri Sud)

  • Silvia Senatore

    (Istituto di Ricerche Farmacologiche “Mario Negri”, Consorzio Mario Negri Sud)

  • Raffaella Mancini

    (Istituto di Ricerche Farmacologiche “Mario Negri”, Consorzio Mario Negri Sud)

  • Elena V. Polishchuk

    (Istituto di Ricerche Farmacologiche “Mario Negri”, Consorzio Mario Negri Sud)

  • Mario Salmona

    (Istituto di Ricerche Farmacologiche “Mario Negri”)

  • Francesco Facchiano

    (Istituto di Ricerche Farmacologiche “Mario Negri”, Consorzio Mario Negri Sud
    Laboratory of Vascular Pathology, Istituto Dermopatico dell' Immacolata)

  • Koert N. J. Burger

    (Institute of Biomembranes, Utrecht University)

  • Alexander Mironov

    (Istituto di Ricerche Farmacologiche “Mario Negri”, Consorzio Mario Negri Sud)

  • Alberto Luini

    (Istituto di Ricerche Farmacologiche “Mario Negri”, Consorzio Mario Negri Sud)

  • Daniela Corda

    (Istituto di Ricerche Farmacologiche “Mario Negri”, Consorzio Mario Negri Sud)

Abstract

Membrane fission is essential in intracellular transport. Acyl-coenzyme As (acyl-CoAs) are important in lipid remodelling and are required for fission of COPI-coated vesicles1. Here we show that CtBP/BARS, a protein that functions in the dynamics of Golgi tubules2, is an essential component of the fission machinery operating at Golgi tubular networks, including Golgi compartments involved in protein transport and sorting. CtBP/BARS-induced fission was preceded by the formation of constricted sites in Golgi tubules, whose extreme curvature is likely to involve local changes in the membrane lipid composition. We find that CtBP/BARS uses acyl-CoA to selectively catalyse the acylation of lysophosphatidic acid to phosphatidic acid both in pure lipidic systems and in Golgi membranes, and that this reaction is essential for fission. Our results indicate a key role for lipid metabolic pathways in membrane fission.

Suggested Citation

  • Roberto Weigert & Maria Giuseppina Silletta & Stefania Spanò & Gabriele Turacchio & Claudia Cericola & Antonino Colanzi & Silvia Senatore & Raffaella Mancini & Elena V. Polishchuk & Mario Salmona & Fr, 1999. "CtBP/BARS induces fission of Golgi membranes by acylating lysophosphatidic acid," Nature, Nature, vol. 402(6760), pages 429-433, November.
    • Handle: RePEc:nat:nature:v:402:y:1999:i:6760:d:10.1038_46587
    DOI: 10.1038/46587
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