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Nucleosome mobilization catalysed by the yeast SWI/SNF complex

Author

Listed:
  • Iestyn Whitehouse

    (The Wellcome Trust Building)

  • Andrew Flaus

    (The Wellcome Trust Building)

  • Bradley R. Cairns

    (Huntsman Cancer Institute)

  • Malcolm F. White

    (University of Dundee)

  • Jerry L. Workman

    (Howard Hughes Medical Institute, The Pennsylvania State University)

  • Tom Owen-Hughes

    (The Wellcome Trust Building)

Abstract

The generation of a local chromatin topology conducive to transcription is a key step in gene regulation1. The yeast SWI/SNF complex is the founding member of a family of ATP-dependent remodelling activities capable of altering chromatin structure both in vitro and in vivo2. Despite its importance, the pathway by which the SWI/SNF complex disrupts chromatin structure is unknown. Here we use a model system to demonstrate that the yeast SWI/SNF complex can reposition nucleosomes in an ATP-dependent reaction that favours attachment of the histone octamer to an acceptor site on the same molecule of DNA (in cis). We show that SWI/SNF-mediated displacement of the histone octamer is effectively blocked by a barrier introduced into the DNA, suggesting that this redistribution involves sliding or tracking of nucleosomes along DNA, and that it is achieved by a catalytic mechanism. We conclude that SWI/SNF catalyses the redistribution of nucleosomes along DNA in cis, which may represent a general mechanism by which ATP-dependent chromatin remodelling occurs.

Suggested Citation

  • Iestyn Whitehouse & Andrew Flaus & Bradley R. Cairns & Malcolm F. White & Jerry L. Workman & Tom Owen-Hughes, 1999. "Nucleosome mobilization catalysed by the yeast SWI/SNF complex," Nature, Nature, vol. 400(6746), pages 784-787, August.
    • Handle: RePEc:nat:nature:v:400:y:1999:i:6746:d:10.1038_23506
    DOI: 10.1038/23506
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    Cited by:

    1. Hyelim Jo & Taemook Kim & Yujin Chun & Inkyung Jung & Daeyoup Lee, 2021. "A compendium of chromatin contact maps reflecting regulation by chromatin remodelers in budding yeast," Nature Communications, Nature, vol. 12(1), pages 1-11, December.

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