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Structure of cytochrome c nitrite reductase

Author

Listed:
  • Oliver Einsle

    (Max-Planck-Institut für Biochemie
    Universität Konstanz, Fakultät für Biologie)

  • Albrecht Messerschmidt

    (Max-Planck-Institut für Biochemie)

  • Petra Stach

    (Universität Konstanz, Fakultät für Biologie)

  • Gleb P. Bourenkov

    (MPG-ASMB c/o DESY, Arbeitsgruppe Proteindynamik)

  • Hans D. Bartunik

    (MPG-ASMB c/o DESY, Arbeitsgruppe Proteindynamik)

  • Robert Huber

    (Max-Planck-Institut für Biochemie)

  • Peter M. H. Kroneck

    (Universität Konstanz, Fakultät für Biologie)

Abstract

The enzyme cytochrome c nitrite reductase catalyses the six-electron reduction of nitrite to ammonia as one of the key stepsin the biological nitrogen cycle1, where it participates inthe anaerobic energy metabolism of dissimilatory nitrate ammonification2. Here we report on the crystal structure of this enzyme from the microorganism Sulfurospirillum deleyianum, which we solved by multiwavelength anomalous dispersion methods. We propose a reaction scheme for the transformation of nitrite based on structural and spectroscopic information. Cytochrome c nitrite reductase is a functional dimer, with 10 close-packed haem groups of type c and an unusual lysine-coordinated high-spin haem at the active site. By comparing the haem arrangement of this nitrite reductase with that of other multihaem cytochromes, we have been able to identify a family of proteins in which the orientation of haem groups is conserved whereas structure and function are not.

Suggested Citation

  • Oliver Einsle & Albrecht Messerschmidt & Petra Stach & Gleb P. Bourenkov & Hans D. Bartunik & Robert Huber & Peter M. H. Kroneck, 1999. "Structure of cytochrome c nitrite reductase," Nature, Nature, vol. 400(6743), pages 476-480, July.
    • Handle: RePEc:nat:nature:v:400:y:1999:i:6743:d:10.1038_22802
    DOI: 10.1038/22802
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    Cited by:

    1. Aurélien Saghaï & Grace Pold & Christopher M. Jones & Sara Hallin, 2023. "Phyloecology of nitrate ammonifiers and their importance relative to denitrifiers in global terrestrial biomes," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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