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Regions of variant histone His2AvD required for Drosophila development

Author

Listed:
  • Michael John Clarkson

    (The John Curtin School of Medical Research, the Australian National University
    Department of Biochemistry)

  • Julian R. E. Wells

    (Department of Biochemistry)

  • Frank Gibson

    (The John Curtin School of Medical Research, the Australian National University)

  • Robert Saint

    (University of Adelaide)

  • David John Tremethick

    (The John Curtin School of Medical Research, the Australian National University)

Abstract

One way in which a distinct chromosomal domain could be established to carry out a specialized function is by the localized incorporation of specific histone variants into nucleosomes. H2AZ, one such variant of the histone protein H2A, is required for the survival of Drosophila melanogaster1, Tetrahymena thermophila2 and mice (R. Faast et al., in preparation). To search for the unique features of Drosophila H2AZ (His2AvD, also referred to as H2AvD) that are required for its essential function, we have performed amino-acid swap experiments in which residues unique to Drosophila His2AvD were replaced with equivalently positioned Drosophila H2A.1 residues. Mutated His2AvD genes encoding modified versions of this histone were transformed into Drosophila and tested for their ability to rescue null-mutant lethality. We show that the unique feature of His2AvD does not reside in its histone fold but in its carboxy-terminal domain. This C-terminal region maps to a short α-helix in H2A that is buried deep inside the nucleosome core.

Suggested Citation

  • Michael John Clarkson & Julian R. E. Wells & Frank Gibson & Robert Saint & David John Tremethick, 1999. "Regions of variant histone His2AvD required for Drosophila development," Nature, Nature, vol. 399(6737), pages 694-697, June.
    • Handle: RePEc:nat:nature:v:399:y:1999:i:6737:d:10.1038_21436
    DOI: 10.1038/21436
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