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K+ is an endothelium-derived hyperpolarizing factor in rat arteries

Author

Listed:
  • G. Edwards

    (Pharmacology and Toxicology, School of Biological Sciences, G38 Stopford Building, University of Manchester)

  • K. A. Dora

    (University of Bristol)

  • M. J. Gardener

    (Pharmacology and Toxicology, School of Biological Sciences, G38 Stopford Building, University of Manchester)

  • C. J. Garland

    (University of Bristol)

  • A. H. Weston

    (Pharmacology and Toxicology, School of Biological Sciences, G38 Stopford Building, University of Manchester)

Abstract

In arteries, muscarinic agonists such as acetylcholine release an unidentified, endothelium-derived hyperpolarizing factor (EDHF) which is neither prostacyclin nor nitric oxide1,2,3. Here we show that EDHF-induced hyperpolarization of smooth muscle and relaxation of small resistance arteries are inhibited by ouabain plus Ba2+; ouabain is a blocker of Na+/K+ ATPase4 and Ba2+ blocks inwardly rectifying K+ channels5. Small increases in the amount of extracellular K+ mimic these effects of EDHF in a ouabain- and Ba2+-sensitive, but endothelium-independent, manner. Acetylcholine hyperpolarizes endothelial cells and increases the K+ concentration in the myoendothelial space; these effects are abolished by charbdotoxin plus apamin. Hyperpolarization of smooth muscle by EDHF is also abolished by this toxin combination, but these toxins do not affect the hyperpolarizaiton of smooth muscle by added K+. These data show that EDHF is K+ that effluxes through charybdotoxin- and apamin-sensitive K+ channels on endothelial cells. The resulting increase in myoendothelial K+ concentration hyperpolarizes and relaxes adjacent smooth-muscle cells by activating Ba2+-sensitive K+ channels and Na+/K+ ATPase. These results show that fluctuations in K+ levels originating within the blood vessel itself are important in regulating mammalian blood pressure and flow.

Suggested Citation

  • G. Edwards & K. A. Dora & M. J. Gardener & C. J. Garland & A. H. Weston, 1998. "K+ is an endothelium-derived hyperpolarizing factor in rat arteries," Nature, Nature, vol. 396(6708), pages 269-272, November.
    • Handle: RePEc:nat:nature:v:396:y:1998:i:6708:d:10.1038_24388
    DOI: 10.1038/24388
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    Cited by:

    1. Anne Marijn van der Graaf & Marjon J Wiegman & Torsten Plösch & Gerda G Zeeman & Azuwerus van Buiten & Robert H Henning & Hendrik Buikema & Marijke M Faas, 2013. "Endothelium-Dependent Relaxation and Angiotensin II Sensitivity in Experimental Preeclampsia," PLOS ONE, Public Library of Science, vol. 8(11), pages 1-1, November.

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