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Mechanism of calcium gating in small-conductance calcium-activated potassium channels

Author

Listed:
  • X.-M. Xia

    (Vollum Institute, Oregon Health Sciences University)

  • B. Fakler

    (University of Tuebingen)

  • A. Rivard

    (Vollum Institute, Oregon Health Sciences University)

  • G. Wayman

    (Vollum Institute, Oregon Health Sciences University)

  • T. Johnson-Pais

    (Vollum Institute, Oregon Health Sciences University)

  • J. E. Keen

    (Vollum Institute, Oregon Health Sciences University)

  • T. Ishii

    (Vollum Institute, Oregon Health Sciences University)

  • B. Hirschberg

    (Vollum Institute, Oregon Health Sciences University)

  • C. T. Bond

    (Vollum Institute, Oregon Health Sciences University)

  • S. Lutsenko

    (Biochemsitry and Molecular Biology, Oregon Health Sciences University)

  • J. Maylie

    (Obstetrics and Gynecology, Oregon Health Sciences University)

  • J. P. Adelman

    (Vollum Institute, Oregon Health Sciences University)

Abstract

The slow afterhyperpolarization that follows an action potential is generated by the activation of small-conductance calcium-activated potassium channels (SK channels). The slow afterhyperpolarization limits the firing frequency of repetitive action potentials (spike-frequency adaption) and is essential for normal neurotransmission1,2,3. SK channels are voltage-independent and activated by submicromolar concentrations of intracellular calcium1. They are high-affinity calcium sensors that transduce fluctuations in intracellular calcium concentrations into changes in membrane potential. Here we study the mechanism of calcium gating and find that SK channels are not gated by calcium binding directly to the channel α-subunits. Instead, the functional SK channels are heteromeric complexes with calmodulin, which is constitutively associated with the α-subunits in a calcium-independent manner. Our data support a model in which calcium gating of SK channels is mediated by binding of calcium to calmodulin and subsequent conformational alterations in the channel protein.

Suggested Citation

  • X.-M. Xia & B. Fakler & A. Rivard & G. Wayman & T. Johnson-Pais & J. E. Keen & T. Ishii & B. Hirschberg & C. T. Bond & S. Lutsenko & J. Maylie & J. P. Adelman, 1998. "Mechanism of calcium gating in small-conductance calcium-activated potassium channels," Nature, Nature, vol. 395(6701), pages 503-507, October.
    • Handle: RePEc:nat:nature:v:395:y:1998:i:6701:d:10.1038_26758
    DOI: 10.1038/26758
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    Cited by:

    1. Erick Olivares & Simón Salgado & Jean Paul Maidana & Gaspar Herrera & Matías Campos & Rodolfo Madrid & Patricio Orio, 2015. "TRPM8-Dependent Dynamic Response in a Mathematical Model of Cold Thermoreceptor," PLOS ONE, Public Library of Science, vol. 10(10), pages 1-17, October.

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