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Induction of the zebrafish ventral brain and floorplate requires cyclops/nodal signalling

Author

Listed:
  • Karuna Sampath

    (Vanderbilt University School of Medicine
    Institute of Molecular Agrobiology, National University of Singapore)

  • Amy L. Rubinstein

    (Carnegie Institution of Washington)

  • Abby M. S. Cheng

    (Vanderbilt University School of Medicine)

  • Jennifer O. Liang

    (Carnegie Institution of Washington)

  • Kimberly Fekany

    (Vanderbilt University)

  • Lilianna Solnica-Krezel

    (Vanderbilt University)

  • Vladimir Korzh

    (Institute of Molecular Agrobiology, National University of Singapore)

  • Marnie E. Halpern

    (Carnegie Institution of Washington)

  • Christopher V. E. Wright

    (Vanderbilt University School of Medicine)

Abstract

Zebrafish cyclops (cyc) mutations cause deficiencies in the dorsal mesendoderm1,2 and ventral neural tube3,4, leading to neural defects and cyclopia5,6. Here we report that cyc encodes a transforming growth factor-β (TGF-β)-related intercellular signalling molecule that is similar to mouse nodal7. cyc is expressed in dorsal mesendoderm at gastrulation and in the prechordal plate until early somitogenesis. Expression reappears transiently in the left lateral-plate mesoderm, and in an unprecedented asymmetric pattern in the left forebrain. Injection of cyc RNA non-autonomously restores sonic hedgehog -expressing cells of the ventral brain and floorplate that are absent in cyc mutants, whereas inducing activities are abolished by cycm294, a mutation of a conserved cysteine in the mature ligand. Our results indicate that cyc provides an essential non-cell-autonomous signal at gastrulation, leading to induction of the floorplate and ventral brain.

Suggested Citation

  • Karuna Sampath & Amy L. Rubinstein & Abby M. S. Cheng & Jennifer O. Liang & Kimberly Fekany & Lilianna Solnica-Krezel & Vladimir Korzh & Marnie E. Halpern & Christopher V. E. Wright, 1998. "Induction of the zebrafish ventral brain and floorplate requires cyclops/nodal signalling," Nature, Nature, vol. 395(6698), pages 185-189, September.
    • Handle: RePEc:nat:nature:v:395:y:1998:i:6698:d:10.1038_26020
    DOI: 10.1038/26020
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