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Male-to-female sex reversal in M33 mutant mice

Author

Listed:
  • Yuko Katoh-Fukui

    (Mitsubishi Kasei Institute of Life Sciences)

  • Reiko Tsuchiya

    (Mitsubishi Kasei Institute of Life Sciences)

  • Toshihiko Shiroishi

    (Mammalian Genetics Laboratory, National Institute of Genetics)

  • Yoko Nakahara

    (Mitsubishi Kasei Institute of Life Sciences)

  • Naoko Hashimoto

    (Mitsubishi Kasei Institute of Life Sciences)

  • Kousei Noguchi

    (Waseda University)

  • Toru Higashinakagawa

    (Waseda University)

Abstract

Polycomb genes in Drosophila maintain the repressed state of homeotic and other developmentally regulated genes1,2,3,4 by mediating changes in higher-order chromatin structure5,6,7. M33, a mouse homologue of Polycomb, was isolated by means of the structural similarity of its chromodomain8. The fifth exon of M33 contains a region of homology shared by Drosophila and Xenopus8,9. In Drosophila, its deletion results in the loss of Polycomb function10. Here we have disrupted M33 in mice by inserting a poly(A) capture-type neor targeting vector into its fifth exon. More than half of the resultant M33cterm/M33cterm mutant mice died before weaning, and survivors showed male-to-female sex reversal. Formation of genital ridges was retarded in both XX and XY M33cterm/M33cterm embryos. Gonadal growth defects appeared near the time of expression of the Y-chromosome-specific Sry gene11, suggesting that M33 deficiency may cause sex reversal by interfering with steps upstream of Sry. M33cterm/M33cterm mice may be a valuable model in which to test opposing views regarding sex determination.

Suggested Citation

  • Yuko Katoh-Fukui & Reiko Tsuchiya & Toshihiko Shiroishi & Yoko Nakahara & Naoko Hashimoto & Kousei Noguchi & Toru Higashinakagawa, 1998. "Male-to-female sex reversal in M33 mutant mice," Nature, Nature, vol. 393(6686), pages 688-692, June.
    • Handle: RePEc:nat:nature:v:393:y:1998:i:6686:d:10.1038_31482
    DOI: 10.1038/31482
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    Cited by:

    1. Daniel Bsteh & Hagar F. Moussa & Georg Michlits & Ramesh Yelagandula & Jingkui Wang & Ulrich Elling & Oliver Bell, 2023. "Loss of cohesin regulator PDS5A reveals repressive role of Polycomb loops," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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