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Function of TAFII-containing complex without TBP in transcription by RNA polymerase II

Author

Listed:
  • Elzbieta Wieczorek

    (Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP
    Virginia Commonwealth University)

  • Marjorie Brand

    (Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP)

  • Xavier Jacq

    (Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP
    Columbia University)

  • László Tora

    (Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP)

Abstract

Initiation of transcription of a gene from a core promoter region by RNA polymerase II requires the assembly of several initiation factors to form a preinitiation complex. Assembly of this complex1,2 is thought to be nucleated exclusively by the sequence-specific binding of the TFIID transcription factor complex, which is composed of the TATA-binding protein (TBP) and TBP-associated factors (TAFIIs) (refs 3, 4), to the different promoters. Here we isolate and characterize a new multiprotein complex that does not contain either TBP or a TBP-like factor but is composed of several TAFIIs and other proteins. This complex can replace TFIID on both TATA-containing and TATA-lacking promoters in in vitro transcription assays. Moreover, an anti-TBP antibody that inhibits TBP- and TFIID-dependent transcription does not inhibit activity of this new complex. These results indicate that TBP-free RNA polymerase II mediated transcription may be able to occur in mammalian cells and that multiple preinitiation complexes may play an important role in regulating gene expression.

Suggested Citation

  • Elzbieta Wieczorek & Marjorie Brand & Xavier Jacq & László Tora, 1998. "Function of TAFII-containing complex without TBP in transcription by RNA polymerase II," Nature, Nature, vol. 393(6681), pages 187-191, May.
    • Handle: RePEc:nat:nature:v:393:y:1998:i:6681:d:10.1038_30283
    DOI: 10.1038/30283
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