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Molecular characterization of a neuronal low-voltage-activated T-type calcium channel

Author

Listed:
  • Edward Perez-Reyes

    (Loyola University Medical Center
    Cardiovascular Institute, Loyola University Medical Center)

  • Leanne L. Cribbs

    (Loyola University Medical Center
    Cardiovascular Institute, Loyola University Medical Center)

  • Asif Daud

    (Loyola University Medical Center)

  • Antonio E. Lacerda

    (Rammelkamp Center for Research & Education, MetroHealth Medical Center)

  • Jane Barclay

    (The Rayne Institute, University College London Medical School)

  • Magali P. Williamson

    (The Rayne Institute, University College London Medical School)

  • Margaret Fox

    (MRC Human Biochemical Genetics Unit, The Galton Laboratory)

  • Michele Rees

    (The Rayne Institute, University College London Medical School)

  • Jung-Ha Lee

    (Loyola University Medical Center)

Abstract

The molecular diversity of voltage-activated calcium channels was established by studies showing that channels could be distinguished by their voltage-dependence, deactivation and single-channel conductance1,2,3. Low-voltage-activated channels are called ‘T’ type because their currents are both transient (owing to fast inactivation) and tiny (owing to small conductance)2. T-type channels are thought to be involved in pacemaker activity, low-threshold calcium spikes, neuronal oscillations and resonance, and rebound burst firing4. Here we report the identification of a neuronal T-type channel. Our cloning strategy began with an analysis of Genbank sequences defined as sharing homology with calcium channels. We sequenced an expressed sequence tag (EST), then used it to clone a full-length complementary DNA from rat brain. Northern blot analysis indicated that this gene is expressed predominantly in brain, in particular the amygdala, cerebellum and thalamus. We mapped the human gene to chromosome 17q22, and the mouse gene to chromosome 11. Functional expression of the channel was measured in Xenopus oocytes. Based on the channel's distinctive voltage dependence, slow deactivation kinetics, and 7.5-pS single-channel conductance, we conclude that this channel is a low-voltage-activated T-type calcium channel.

Suggested Citation

  • Edward Perez-Reyes & Leanne L. Cribbs & Asif Daud & Antonio E. Lacerda & Jane Barclay & Magali P. Williamson & Margaret Fox & Michele Rees & Jung-Ha Lee, 1998. "Molecular characterization of a neuronal low-voltage-activated T-type calcium channel," Nature, Nature, vol. 391(6670), pages 896-900, February.
  • Handle: RePEc:nat:nature:v:391:y:1998:i:6670:d:10.1038_36110
    DOI: 10.1038/36110
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    Cited by:

    1. Lingli He & Zhuoya Yu & Ze Geng & Zhuo Huang & Changjiang Zhang & Yanli Dong & Yiwei Gao & Yuhang Wang & Qihao Chen & Le Sun & Xinyue Ma & Bo Huang & Xiaoqun Wang & Yan Zhao, 2022. "Structure, gating, and pharmacology of human CaV3.3 channel," Nature Communications, Nature, vol. 13(1), pages 1-9, December.

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