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COPII–cargo interactions direct protein sorting into ER-derived transport vesicles

Author

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  • Meta J. Kuehn

    (Barker Hall, Howard Hughes Medical Institute, University of California)

  • Johannes M. Herrmann

    (Barker Hall, Howard Hughes Medical Institute, University of California)

  • Randy Schekman

    (Barker Hall, Howard Hughes Medical Institute, University of California)

Abstract

Vesicles coated with coat protein complex II (COPII) selectively transport molecules (cargo) and vesicle fusion proteins from the endoplasmic reticulum (ER) to the Golgi complex1,2,3. We have investigated the role of coat proteins in cargo selection and recruitment. We isolated integral membrane and soluble cargo proteins destined for transport from the ER in complexes formed in the presence of Sar1 and Sec23/24, a subset of the COPII components, and GTP or GMP-PNP. Vesicle fusion proteins of the vSNARE family and Emp24, a member of a putative cargo carrier family4, were also found in COPII complexes. The inclusion of amino-acid permease molecules into the complex depended on the presence of Shr3, a protein required for the permease to leave the ER3,5. Resident ER proteins Sec61, BiP (Kar2) and Shr3 were not included in the complexes, indicating that the COPII components bound specifically to vesicle cargo. COPII–cargo complexes and putative cargo adaptor–cargo complexes were also isolated from COPII vesicles. Our results indicate that cargo packaging signals and soluble cargo adaptors are recognized by a recruitment complex comprising Sar1–GTP and Sec23/24.

Suggested Citation

  • Meta J. Kuehn & Johannes M. Herrmann & Randy Schekman, 1998. "COPII–cargo interactions direct protein sorting into ER-derived transport vesicles," Nature, Nature, vol. 391(6663), pages 187-190, January.
    • Handle: RePEc:nat:nature:v:391:y:1998:i:6663:d:10.1038_34438
    DOI: 10.1038/34438
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    Cited by:

    1. Julia D. Knopf & Susanne S. Steigleder & Friederike Korn & Nathalie Kühnle & Marina Badenes & Marina Tauber & Sebastian J. Theobald & Jan Rybniker & Colin Adrain & Marius K. Lemberg, 2024. "RHBDL4-triggered downregulation of COPII adaptor protein TMED7 suppresses TLR4-mediated inflammatory signaling," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    2. William Kasberg & Peter Luong & Kevin A. Swift & Anjon Audhya, 2023. "Nutrient deprivation alters the rate of COPII subunit recruitment at ER subdomains to tune secretory protein transport," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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