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Drosophila Mad binds to DNA and directly mediates activation of vestigial by Decapentaplegic

Author

Listed:
  • Jaeseob Kim

    (*Howard Hughes Medical Institute and Laboratory of Molecular Biology, University of Wisconsin)

  • Kirby Johnson

    (445 Henry Mall, University of Wisconsin-Madison)

  • Hui Ju Chen

    (McArdle Laboratory, University of Wisconsin-Madison)

  • Sean Carroll

    (*Howard Hughes Medical Institute and Laboratory of Molecular Biology, University of Wisconsin
    445 Henry Mall, University of Wisconsin-Madison)

  • Allen Laughon

    (Correspondence and requests for materials should be addressed to A.L.)

Abstract

The TGF-β (transforming growth factor-β)-related signalling proteins, including Decapentaplegic (Dpp) in Drosophila and bone morphogenic proteins and activin in vertebrates, affect the growth and patterning of a great variety of structures. However, the mechanisms by which these ligands regulate gene expression are not understood. Activation of complexes of type I with type II receptors results in the phosphorylation and nuclear localization of members of the SMAD protein family1,2,3,4,5,6,7,8,9, which are thought to act as co-activators of transcription, perhaps in conjunction with sequence-specific cofactors10. Here we show that the amino-terminal domain of the Drosophila Mothers against dpp protein (Mad), a mediator of Dpp signalling11,12,13,14, possesses a sequence-specific DNA-binding activity that becomes apparent when carboxy-terminal residues are removed. Mad binds to and is required for the activation of an enhancer within the vestigial wing-patterning gene in cells across the entire developing wing blade. Mad also binds to Dpp-response elements in other genes. These results suggest that Dpp signalling regulates gene expression by activating Mad binding to target gene enhancers.

Suggested Citation

  • Jaeseob Kim & Kirby Johnson & Hui Ju Chen & Sean Carroll & Allen Laughon, 1997. "Drosophila Mad binds to DNA and directly mediates activation of vestigial by Decapentaplegic," Nature, Nature, vol. 388(6639), pages 304-308, July.
    • Handle: RePEc:nat:nature:v:388:y:1997:i:6639:d:10.1038_40906
    DOI: 10.1038/40906
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    Cited by:

    1. Shinya Matsuda & Jonas V. Schaefer & Yusuke Mii & Yutaro Hori & Dimitri Bieli & Masanori Taira & Andreas Plückthun & Markus Affolter, 2021. "Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc," Nature Communications, Nature, vol. 12(1), pages 1-18, December.

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