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The nucleotide sequence of Saccharomyces cerevisiae chromosome IX

Author

Listed:
  • C. Churcher

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • S. Bowman

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • K. Badcock

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • A. Bankier

    (MRC Laboratory of Molecular Biology)

  • D. Brown

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • T. Chillingworth

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • R. Connor

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • K. Devlin

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • S. Gentles

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • N. Hamlin

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • D. Harris

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • T. Horsnell

    (MRC Laboratory of Molecular Biology)

  • S. Hunt

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • K. Jagels

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • M. Jones

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • G. Lye

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • S. Moule

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • C. Odell

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • D. Pearson

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • M. Rajandream

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • P. Rice

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • N. Rowley

    (MRC Laboratory of Molecular Biology)

  • J. Skelton

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • V. Smith

    (MRC Laboratory of Molecular Biology)

  • S. Walsh

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • S. Whitehead

    (The Sanger Centre, Wellcome Trust Genome Campus)

  • B. Barrell

    (The Sanger Centre, Wellcome Trust Genome Campus)

Abstract

Large-scale systematic sequencing has generally depended on the availability of an ordered library of large-insert bacterial or viral genomic clones for the organism under study. The generation of these large insert libraries, and the location of each clone on a genome map, is a laborious and time-consuming process. In an effort to overcome these problems, several groups have successfully demonstrated the viability of the whole-genome random ‘shotgun’ method in large-scale sequencing of both viruses and prokaryotes1–5. Here we report the sequence of Saccharomyces cerevisiae chromosome IX, determined in part by a whole-chromosome ‘shotgun’, and describe the particular difficulties encountered in the random ‘shotgun’ sequencing of an entire eukaryotic chromosome. Analysis of this sequence shows that chromosome IX contains 221 open reading frames (ORFs), of which approximately 30% have been sequenced previously. This chromosome shows features typical of a small Saccharomyces cerevisiae chromosome.

Suggested Citation

  • C. Churcher & S. Bowman & K. Badcock & A. Bankier & D. Brown & T. Chillingworth & R. Connor & K. Devlin & S. Gentles & N. Hamlin & D. Harris & T. Horsnell & S. Hunt & K. Jagels & M. Jones & G. Lye & S, 1997. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX," Nature, Nature, vol. 387(6632), pages 84-87, May.
    • Handle: RePEc:nat:nature:v:387:y:1997:i:6632:d:10.1038_387s084
    DOI: 10.1038/387s084
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