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Activation of prokaryotic transcription through arbitrary protein–protein contacts

Author

Listed:
  • Simon L. Dove

    (Harvard Medical School)

  • J. Keith Joung

    (Harvard Medical School
    Massachusetts General Hospital)

  • Ann Hochschild

    (Harvard Medical School)

Abstract

Many transcriptional activators in prokaryotes are known to bind near a promoter and contact RNA polymerase1–5, but it is not clear whether a protein–protein contact between an activator and RNA polymerase is enough to activate gene transcription. Here we show that contact between a DNA-bound protein and a heterologous protein domain fused to RNA polymerase can elicit transcriptional activation; moreover, the strength of this engineered protein–protein interaction determines the amount of gene activation. Our results indicate that an arbitrary interaction between a DNA-bound protein and RNA polymerase can activate transcription. We also find that when the DNA-bound 'activator' makes contact with two different components of the polymerase, the effect of these two interactions on transcription is synergistic.

Suggested Citation

  • Simon L. Dove & J. Keith Joung & Ann Hochschild, 1997. "Activation of prokaryotic transcription through arbitrary protein–protein contacts," Nature, Nature, vol. 386(6625), pages 627-630, April.
    • Handle: RePEc:nat:nature:v:386:y:1997:i:6625:d:10.1038_386627a0
    DOI: 10.1038/386627a0
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    Cited by:

    1. Mattias Rydenfelt & Hernan G Garcia & Robert Sidney Cox III & Rob Phillips, 2014. "The Influence of Promoter Architectures and Regulatory Motifs on Gene Expression in Escherichia coli," PLOS ONE, Public Library of Science, vol. 9(12), pages 1-31, December.

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