IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v385y1997i6614d10.1038_385336a0.html
   My bibliography  Save this article

Circumsporozoite protein is required for development of malaria sporozoites in mosquitoes

Author

Listed:
  • Robert Ménard

    (New York University Medical Center
    Department of Medical and Molecular Parasitology)

  • Ali A. Sultan

    (New York University Medical Center)

  • Claudio Cortes

    (Department of Medical and Molecular Parasitology
    University Medical Center)

  • Rita Altszuler

    (Department of Medical and Molecular Parasitology
    University Medical Center)

  • Melissa R. van Dijk

    (University of Leiden)

  • Chris J. Janse

    (University of Leiden)

  • Andrew P. Waters

    (University of Leiden)

  • Ruth S. Nussenzweig

    (Department of Medical and Molecular Parasitology
    University Medical Center)

  • Victor Nussenzweig

    (New York University Medical Center)

Abstract

Malaria parasites undergo a sporogonic cycle in the mosquito vector. Sporozoites, the form of the parasite injected into the host during a bloodmeal, develop inside oocysts in the insect midgut, then migrate to and eventually invade the salivary glands. The circumsporozoite protein (CS), one of the major proteins synthesized by salivary gland sporozoites1, is a surface-associated molecule which is important in sporozoite infectivity to the host2. Here, by gene targeting, we created Plasmodium berghei lines in which the single-copy CS gene was disrupted. The CS( − ) and wild-type parasites produced similar numbers of oocysts of comparable size in the mosquito midgut. In the CS( − ) oocysts, however, sporozoite formation was profoundly inhibited. CS therefore appears to have a pleiotropic role and to be vital for malaria parasites in both the vector and the host: in mosquitoes, CS is essential for sporozoite development within oocysts, and in the vertebrate host it promotes sporozoite attachment to hepatocytes3–7.

Suggested Citation

  • Robert Ménard & Ali A. Sultan & Claudio Cortes & Rita Altszuler & Melissa R. van Dijk & Chris J. Janse & Andrew P. Waters & Ruth S. Nussenzweig & Victor Nussenzweig, 1997. "Circumsporozoite protein is required for development of malaria sporozoites in mosquitoes," Nature, Nature, vol. 385(6614), pages 336-340, January.
    • Handle: RePEc:nat:nature:v:385:y:1997:i:6614:d:10.1038_385336a0
    DOI: 10.1038/385336a0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/385336a0
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/385336a0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Feng Zhu & Hong Zheng & Suilin Chen & Kun Zhang & Xin Qin & Jingru Zhang & Taiping liu & Yongling Fan & Liting Wang & Xiaoxu Li & Jian Zhang & Wenyue Xu, 2022. "Malaria oocysts require circumsporozoite protein to evade mosquito immunity," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:385:y:1997:i:6614:d:10.1038_385336a0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.