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The ASCIZ-DYNLL1 axis promotes 53BP1-dependent non-homologous end joining and PARP inhibitor sensitivity

Author

Listed:
  • Jordan R. Becker

    (University of Oxford)

  • Raquel Cuella-Martin

    (University of Oxford)

  • Marco Barazas

    (The Netherlands Cancer Institute)

  • Rui Liu

    (St. Vincent’s Institute of Medical Research)

  • Catarina Oliveira

    (University of Oxford)

  • Antony W. Oliver

    (University of Sussex)

  • Kirstin Bilham

    (University of Oxford)

  • Abbey B. Holt

    (University of Oxford)

  • Andrew N. Blackford

    (University of Oxford, John Radcliffe Hospital
    University of Oxford)

  • Jörg Heierhorst

    (St. Vincent’s Institute of Medical Research
    The University of Melbourne)

  • Jos Jonkers

    (The Netherlands Cancer Institute)

  • Sven Rottenberg

    (The Netherlands Cancer Institute
    University of Bern)

  • J. Ross Chapman

    (University of Oxford)

Abstract

53BP1 controls a specialized non-homologous end joining (NHEJ) pathway that is essential for adaptive immunity, yet oncogenic in BRCA1 mutant cancers. Intra-chromosomal DNA double-strand break (DSB) joining events during immunoglobulin class switch recombination (CSR) require 53BP1. However, in BRCA1 mutant cells, 53BP1 blocks homologous recombination (HR) and promotes toxic NHEJ, resulting in genomic instability. Here, we identify the protein dimerization hub—DYNLL1—as an organizer of multimeric 53BP1 complexes. DYNLL1 binding stimulates 53BP1 oligomerization, and promotes 53BP1’s recruitment to, and interaction with, DSB-associated chromatin. Consequently, DYNLL1 regulates 53BP1-dependent NHEJ: CSR is compromised upon deletion of Dynll1 or its transcriptional regulator Asciz, or by mutation of DYNLL1 binding motifs in 53BP1; furthermore, Brca1 mutant cells and tumours are rendered resistant to poly-ADP ribose polymerase (PARP) inhibitor treatments upon deletion of Dynll1 or Asciz. Thus, our results reveal a mechanism that regulates 53BP1-dependent NHEJ and the therapeutic response of BRCA1-deficient cancers.

Suggested Citation

  • Jordan R. Becker & Raquel Cuella-Martin & Marco Barazas & Rui Liu & Catarina Oliveira & Antony W. Oliver & Kirstin Bilham & Abbey B. Holt & Andrew N. Blackford & Jörg Heierhorst & Jos Jonkers & Sven R, 2018. "The ASCIZ-DYNLL1 axis promotes 53BP1-dependent non-homologous end joining and PARP inhibitor sensitivity," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-07855-x
    DOI: 10.1038/s41467-018-07855-x
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