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Functional genomic analysis identifies miRNA repertoire regulating C. elegans oocyte development

Author

Listed:
  • Amanda L. Minogue

    (Baylor College of Medicine
    U.T. MD Anderson Cancer Center)

  • Michael R. Tackett

    (Abcam, One Kendall Square)

  • Elnaz Atabakhsh

    (Abcam, One Kendall Square)

  • Genesis Tejada

    (Abcam, One Kendall Square)

  • Swathi Arur

    (Baylor College of Medicine
    U.T. MD Anderson Cancer Center)

Abstract

Oocyte-specific miRNA function remains unclear in mice and worms because loss of Dgcr8 and Dicer from mouse and worm oocytes, respectively, does not yield oogenic defects. These data lead to several models: (a) miRNAs are not generated in oocytes; (b) miRNAs are generated but do not perform an oogenic function; (c) functional oocyte miRNAs are generated in a manner independent of these enzymes. Here, we test these models using a combination of genomic, expression and functional analyses on the C. elegans germline. We identify a repertoire of at least twenty-three miRNAs that accumulate in four spatial domains in oocytes. Genetic tests demonstrate that oocyte-expressed miRNAs regulate key oogenic processes within their respective expression domains. Unexpectedly, we find that over half of the oocyte-expressed miRNAs are generated through an unknown Drosha independent mechanism. Thus, a functional miRNA repertoire generated via Drosha dependent and independent pathways regulates C. elegans oocyte development.

Suggested Citation

  • Amanda L. Minogue & Michael R. Tackett & Elnaz Atabakhsh & Genesis Tejada & Swathi Arur, 2018. "Functional genomic analysis identifies miRNA repertoire regulating C. elegans oocyte development," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-07791-w
    DOI: 10.1038/s41467-018-07791-w
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    Cited by:

    1. Weina Xu & Jinyi Liu & Huan Qi & Ruolin Si & Zhiguang Zhao & Zhiju Tao & Yuchuan Bai & Shipeng Hu & Xiaohan Sun & Yulin Cong & Haoye Zhang & Duchangjiang Fan & Long Xiao & Yangyang Wang & Yongbin Li &, 2024. "A lineage-resolved cartography of microRNA promoter activity in C. elegans empowers multidimensional developmental analysis," Nature Communications, Nature, vol. 15(1), pages 1-23, December.

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