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Hidden heterogeneity and circadian-controlled cell fate inferred from single cell lineages

Author

Listed:
  • Shaon Chakrabarti

    (Dana-Farber Cancer Institute
    Harvard T. H. Chan School of Public Health
    Harvard University)

  • Andrew L. Paek

    (Harvard Medical School
    University of Arizona)

  • Jose Reyes

    (Harvard Medical School)

  • Kathleen A. Lasick

    (University of Arizona)

  • Galit Lahav

    (Harvard Medical School
    Broad Institute of Harvard and MIT
    Ludwig Center at Harvard)

  • Franziska Michor

    (Dana-Farber Cancer Institute
    Harvard T. H. Chan School of Public Health
    Harvard University
    Broad Institute of Harvard and MIT)

Abstract

The origin of lineage correlations among single cells and the extent of heterogeneity in their intermitotic times (IMT) and apoptosis times (AT) remain incompletely understood. Here we developed single cell lineage-tracking experiments and computational algorithms to uncover correlations and heterogeneity in the IMT and AT of a colon cancer cell line before and during cisplatin treatment. These correlations could not be explained using simple protein production/degradation models. Sister cell fates were similar regardless of whether they divided before or after cisplatin administration and did not arise from proximity-related factors, suggesting fate determination early in a cell’s lifetime. Based on these findings, we developed a theoretical model explaining how the observed correlation structure can arise from oscillatory mechanisms underlying cell fate control. Our model recapitulated the data only with very specific oscillation periods that fit measured circadian rhythms, thereby suggesting an important role of the circadian clock in controlling cellular fates.

Suggested Citation

  • Shaon Chakrabarti & Andrew L. Paek & Jose Reyes & Kathleen A. Lasick & Galit Lahav & Franziska Michor, 2018. "Hidden heterogeneity and circadian-controlled cell fate inferred from single cell lineages," Nature Communications, Nature, vol. 9(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-07788-5
    DOI: 10.1038/s41467-018-07788-5
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