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Monitoring drug nanocarriers in human blood by near-infrared fluorescence correlation spectroscopy

Author

Listed:
  • Inka Negwer

    (Max Planck Institute for Polymer Research
    Pharmaceutical Chemistry, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University)

  • Andreas Best

    (Max Planck Institute for Polymer Research)

  • Meike Schinnerer

    (Institute of Physical Chemistry, Johannes Gutenberg University
    Institute of Organic Chemistry, Johannes Gutenberg University)

  • Olga Schäfer

    (Institute of Organic Chemistry, Johannes Gutenberg University)

  • Leon Capeloa

    (Institute of Organic Chemistry, Johannes Gutenberg University)

  • Manfred Wagner

    (Max Planck Institute for Polymer Research)

  • Manfred Schmidt

    (Institute of Physical Chemistry, Johannes Gutenberg University)

  • Volker Mailänder

    (Max Planck Institute for Polymer Research
    Department of Dermatology, University Medical Center of the Johannes Gutenberg University)

  • Mark Helm

    (Pharmaceutical Chemistry, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University)

  • Matthias Barz

    (Institute of Organic Chemistry, Johannes Gutenberg University)

  • Hans-Jürgen Butt

    (Max Planck Institute for Polymer Research
    Earth-Life Science Institute, Tokyo Institute of Technology, Meguro)

  • Kaloian Koynov

    (Max Planck Institute for Polymer Research)

Abstract

Nanocarrier-based drug delivery is a promising therapeutic approach that offers unique possibilities for the treatment of various diseases. However, inside the blood stream, nanocarriers’ properties may change significantly due to interactions with proteins, aggregation, decomposition or premature loss of cargo. Thus, a method for precise, in situ characterization of drug nanocarriers in blood is needed. Here we show how the fluorescence correlation spectroscopy that is a well-established method for measuring the size, loading efficiency and stability of drug nanocarriers in aqueous solutions can be used to directly characterize drug nanocarriers in flowing blood. As the blood is not transparent for visible light and densely crowded with cells, we label the nanocarriers or their cargo with near-infrared fluorescent dyes and fit the experimental autocorrelation functions with an analytical model accounting for the presence of blood cells. The developed methodology contributes towards quantitative understanding of the in vivo behavior of nanocarrier-based therapeutics.

Suggested Citation

  • Inka Negwer & Andreas Best & Meike Schinnerer & Olga Schäfer & Leon Capeloa & Manfred Wagner & Manfred Schmidt & Volker Mailänder & Mark Helm & Matthias Barz & Hans-Jürgen Butt & Kaloian Koynov, 2018. "Monitoring drug nanocarriers in human blood by near-infrared fluorescence correlation spectroscopy," Nature Communications, Nature, vol. 9(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-07755-0
    DOI: 10.1038/s41467-018-07755-0
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