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Membrane water for probing neuronal membrane potentials and ionic fluxes at the single cell level

Author

Listed:
  • M. E. P. Didier

    (École Polytechnique Fédérale de Lausanne (EPFL))

  • O. B. Tarun

    (École Polytechnique Fédérale de Lausanne (EPFL))

  • P. Jourdain

    (École Polytechnique Fédérale de Lausanne (EPFL))

  • P. Magistretti

    (École Polytechnique Fédérale de Lausanne (EPFL))

  • S. Roke

    (École Polytechnique Fédérale de Lausanne (EPFL))

Abstract

Neurons communicate through electrochemical signaling within a complex network. These signals are composed of changes in membrane potentials and are traditionally measured with the aid of (toxic) fluorescent labels or invasive electrical probes. Here, we demonstrate an improvement in label-free second harmonic neuroimaging sensitivity by ~3 orders of magnitude using a wide-field medium repetition rate illumination. We perform a side-by-side patch-clamp and second harmonic imaging comparison to demonstrate the theoretically predicted linear correlation between whole neuron membrane potential changes and the square root of the second harmonic intensity. We assign the ion induced changes to the second harmonic intensity to changes in the orientation of membrane interfacial water, which is used to image spatiotemporal changes in the membrane potential and K+ ion flux. We observe a non-uniform spatial distribution and temporal activity of ion channels in mouse brain neurons.

Suggested Citation

  • M. E. P. Didier & O. B. Tarun & P. Jourdain & P. Magistretti & S. Roke, 2018. "Membrane water for probing neuronal membrane potentials and ionic fluxes at the single cell level," Nature Communications, Nature, vol. 9(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-07713-w
    DOI: 10.1038/s41467-018-07713-w
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    Cited by:

    1. Yide Zhang & Binglin Shen & Tong Wu & Jerry Zhao & Joseph C. Jing & Peng Wang & Kanomi Sasaki-Capela & William G. Dunphy & David Garrett & Konstantin Maslov & Weiwei Wang & Lihong V. Wang, 2022. "Ultrafast and hypersensitive phase imaging of propagating internodal current flows in myelinated axons and electromagnetic pulses in dielectrics," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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