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TGNap1 is required for microtubule-dependent homeostasis of a subpopulation of the plant trans-Golgi network

Author

Listed:
  • Luciana Renna

    (Michigan State University)

  • Giovanni Stefano

    (Michigan State University
    Michigan State University)

  • Erin Slabaugh

    (Michigan State University
    North Carolina State University)

  • Clarissa Wormsbaecher

    (Michigan State University
    The Ohio State University)

  • Alan Sulpizio

    (Michigan State University
    Cornell University)

  • Krzysztof Zienkiewicz

    (Michigan State University
    Georg-August-University, Albrecht-von-Haller-Institute for Plant Sciences)

  • Federica Brandizzi

    (Michigan State University
    Michigan State University)

Abstract

Defining convergent and divergent mechanisms underlying the biogenesis and function of endomembrane organelles is fundamentally important in cell biology. In all eukaryotes, the Trans-Golgi Network (TGN) is the hub where the exocytic and endocytic pathways converge. To gain knowledge in the mechanisms underlying TGN biogenesis and function, we characterized TGNap1, a protein encoded by a plant gene of unknown function conserved with metazoans. We demonstrate that TGNap1 is a TGN protein required for the homeostasis of biosynthetic and endocytic traffic pathways. We also show that TGNap1 binds Rab6, YIP4 and microtubules. Finally, we establish that TGNap1 contributes to microtubule-dependent biogenesis, tracking and function of a TGN subset, likely through interaction with Rab6 and YIP4. Our results identify an important trafficking determinant at the plant TGN and reveal an unexpected reliance of post-Golgi traffic homeostasis and organelle biogenesis on microtubules in plants.

Suggested Citation

  • Luciana Renna & Giovanni Stefano & Erin Slabaugh & Clarissa Wormsbaecher & Alan Sulpizio & Krzysztof Zienkiewicz & Federica Brandizzi, 2018. "TGNap1 is required for microtubule-dependent homeostasis of a subpopulation of the plant trans-Golgi network," Nature Communications, Nature, vol. 9(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-07662-4
    DOI: 10.1038/s41467-018-07662-4
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    Cited by:

    1. Deepak D. Bhandari & Dae Kwan Ko & Sang-Jin Kim & Kinya Nomura & Sheng Yang He & Federica Brandizzi, 2023. "Defense against phytopathogens relies on efficient antimicrobial protein secretion mediated by the microtubule-binding protein TGNap1," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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