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Efflux pump-mediated resistance to antifungal compounds can be prevented by conjugation with triphenylphosphonium cation

Author

Listed:
  • Wenqiang Chang

    (Shandong University)

  • Jun Liu

    (Shandong University)

  • Ming Zhang

    (Shandong University)

  • Hongzhuo Shi

    (Shandong University)

  • Sha Zheng

    (Shandong University)

  • Xueyang Jin

    (Shandong University)

  • Yanhui Gao

    (Shandong University)

  • Shuqi Wang

    (Shandong University)

  • Aiguo Ji

    (Shandong University)

  • Hongxiang Lou

    (Shandong University)

Abstract

Antifungal resistance due to upregulation of efflux pumps is prevalent in clinical Candida isolates. Potential efflux pump substrates (PEPSs), which are active against strains deficient in efflux pumps but inactive against wild-type strains, are usually missed in routine antifungal screening. Here we present a method for identification of PEPSs, and show that conjugation with mitochondria-targeting triphenylphosphonium cation (TPP+) can enhance or restore the compounds’ antifungal activity. The screening method involves co-culturing a wild-type C. albicans strain and a Cdr efflux pump-deficient strain, labelled with different fluorescent proteins. We identify several PEPSs from a library of natural terpenes, and restore their antifungal activity against wild-type and azole-resistant C. albicans by conjugation with TPP+. The most active conjugate (IS-2-Pi-TPP) kills C. albicans cells, prevents biofilm formation and eliminates preformed biofilms, without inducing significant resistance. The antifungal activity is accompanied by mitochondrial dysfunction and increased levels of intracellular reactive oxygen species. In addition, IS-2-Pi-TPP is effective against C. albicans in a mouse model of skin infection.

Suggested Citation

  • Wenqiang Chang & Jun Liu & Ming Zhang & Hongzhuo Shi & Sha Zheng & Xueyang Jin & Yanhui Gao & Shuqi Wang & Aiguo Ji & Hongxiang Lou, 2018. "Efflux pump-mediated resistance to antifungal compounds can be prevented by conjugation with triphenylphosphonium cation," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-07633-9
    DOI: 10.1038/s41467-018-07633-9
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