IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v9y2018i1d10.1038_s41467-018-07444-y.html
   My bibliography  Save this article

Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5

Author

Listed:
  • Meng Shi

    (Nanyang Technological University)

  • Bing Chen

    (Nanyang Technological University)

  • Divyanshu Mahajan

    (Nanyang Technological University)

  • Boon Kim Boh

    (Nanyang Technological University)

  • Yan Zhou

    (Nanyang Technological University)

  • Bamaprasad Dutta

    (Nanyang Technological University)

  • Hieng Chiong Tie

    (Nanyang Technological University)

  • Siu Kwan Sze

    (Nanyang Technological University)

  • Geng Wu

    (Shanghai Jiao Tong University)

  • Lei Lu

    (Nanyang Technological University)

Abstract

The endosome-to-Golgi or endocytic retrograde trafficking pathway is an important post-Golgi recycling route. Here we show that amino acids (AAs) can stimulate the retrograde trafficking and regulate the cell surface localization of certain Golgi membrane proteins. By testing components of the AA-stimulated mTORC1 signaling pathway, we demonstrate that SLC38A9, v-ATPase and Ragulator, but not Rag GTPases and mTORC1, are essential for the AA-stimulated trafficking. Arl5, an ARF-like family small GTPase, interacts with Ragulator in an AA-regulated manner and both Arl5 and its effector, the Golgi-associated retrograde protein complex (GARP), are required for the AA-stimulated trafficking. We have therefore identified a mechanistic connection between the nutrient signaling and the retrograde trafficking pathway, whereby SLC38A9 and v-ATPase sense AA-sufficiency and Ragulator might function as a guanine nucleotide exchange factor to activate Arl5, which, together with GARP, a tethering factor, probably facilitates the endosome-to-Golgi trafficking.

Suggested Citation

  • Meng Shi & Bing Chen & Divyanshu Mahajan & Boon Kim Boh & Yan Zhou & Bamaprasad Dutta & Hieng Chiong Tie & Siu Kwan Sze & Geng Wu & Lei Lu, 2018. "Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5," Nature Communications, Nature, vol. 9(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-07444-y
    DOI: 10.1038/s41467-018-07444-y
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-018-07444-y
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-018-07444-y?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-07444-y. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.