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Near-infrared STED nanoscopy with an engineered bacterial phytochrome

Author

Listed:
  • Maria Kamper

    (Max Planck Institute for Biophysical Chemistry)

  • Haisen Ta

    (Max Planck Institute for Biophysical Chemistry)

  • Nickels A. Jensen

    (Max Planck Institute for Biophysical Chemistry)

  • Stefan W. Hell

    (Max Planck Institute for Biophysical Chemistry)

  • Stefan Jakobs

    (Max Planck Institute for Biophysical Chemistry
    University Medical Center Göttingen)

Abstract

The near infrared (NIR) optical window between the cutoff for hemoglobin absorption at 650 nm and the onset of increased water absorption at 900 nm is an attractive, yet largely unexplored, spectral regime for diffraction-unlimited super-resolution fluorescence microscopy (nanoscopy). We developed the NIR fluorescent protein SNIFP, a bright and photostable bacteriophytochrome, and demonstrate its use as a fusion tag in live-cell microscopy and STED nanoscopy. We further demonstrate dual color red-confocal/NIR-STED imaging by co-expressing SNIFP with a conventional red fluorescent protein.

Suggested Citation

  • Maria Kamper & Haisen Ta & Nickels A. Jensen & Stefan W. Hell & Stefan Jakobs, 2018. "Near-infrared STED nanoscopy with an engineered bacterial phytochrome," Nature Communications, Nature, vol. 9(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-07246-2
    DOI: 10.1038/s41467-018-07246-2
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