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Thermostable virus portal proteins as reprogrammable adapters for solid-state nanopore sensors

Author

Listed:
  • Benjamin Cressiot

    (Northeastern University
    Northeastern University
    UniversiteĢ Paris-Saclay)

  • Sandra J. Greive

    (University of York)

  • Mehrnaz Mojtabavi

    (Northeastern University)

  • Alfred A. Antson

    (University of York)

  • Meni Wanunu

    (Northeastern University
    Northeastern University)

Abstract

Nanopore-based sensors are advancing the sensitivity and selectivity of single-molecule detection in molecular medicine and biotechnology. Current electrical sensing devices are based on either membrane protein pores supported in planar lipid bilayers or solid-state (SS) pores fabricated in thin metallic membranes. While both types of nanosensors have been used in a variety of applications, each has inherent disadvantages that limit its use. Hybrid nanopores, consisting of a protein pore supported within a SS membrane, combine the robust nature of SS membranes with the precise and simple engineering of protein nanopores. We demonstrate here a novel lipid-free hybrid nanopore comprising a natural DNA pore from a thermostable virus, electrokinetically inserted into a larger nanopore supported in a silicon nitride membrane. The hybrid pore is stable and easy to fabricate, and, most importantly, exhibits low peripheral leakage allowing sensing and discrimination among different types of biomolecules.

Suggested Citation

  • Benjamin Cressiot & Sandra J. Greive & Mehrnaz Mojtabavi & Alfred A. Antson & Meni Wanunu, 2018. "Thermostable virus portal proteins as reprogrammable adapters for solid-state nanopore sensors," Nature Communications, Nature, vol. 9(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-07116-x
    DOI: 10.1038/s41467-018-07116-x
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