IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v9y2018i1d10.1038_s41467-018-06908-5.html
   My bibliography  Save this article

A distinct isoform of ZNF207 controls self-renewal and pluripotency of human embryonic stem cells

Author

Listed:
  • Fang Fang

    (Montana State University
    Montana State University)

  • Ninuo Xia

    (Montana State University
    Montana State University)

  • Benjamin Angulo

    (Montana State University
    Montana State University)

  • Joseph Carey

    (Montana State University
    Montana State University)

  • Zackery Cady

    (Montana State University
    Montana State University)

  • Jens Durruthy-Durruthy

    (Stanford University School of Medicine)

  • Theo Bennett

    (Montana State University
    Montana State University)

  • Vittorio Sebastiano

    (Stanford University School of Medicine)

  • Renee A. Reijo Pera

    (Montana State University
    Montana State University)

Abstract

Self-renewal and pluripotency in human embryonic stem cells (hESCs) depends upon the function of a remarkably small number of master transcription factors (TFs) that include OCT4, SOX2, and NANOG. Endogenous factors that regulate and maintain the expression of master TFs in hESCs remain largely unknown and/or uncharacterized. Here, we use a genome-wide, proteomics approach to identify proteins associated with the OCT4 enhancer. We identify known OCT4 regulators, plus a subset of potential regulators including a zinc finger protein, ZNF207, that plays diverse roles during development. In hESCs, ZNF207 partners with master pluripotency TFs to govern self-renewal and pluripotency while simultaneously controlling commitment of cells towards ectoderm through direct regulation of neuronal TFs, including OTX2. The distinct roles of ZNF207 during differentiation occur via isoform switching. Thus, a distinct isoform of ZNF207 functions in hESCs at the nexus that balances pluripotency and differentiation to ectoderm.

Suggested Citation

  • Fang Fang & Ninuo Xia & Benjamin Angulo & Joseph Carey & Zackery Cady & Jens Durruthy-Durruthy & Theo Bennett & Vittorio Sebastiano & Renee A. Reijo Pera, 2018. "A distinct isoform of ZNF207 controls self-renewal and pluripotency of human embryonic stem cells," Nature Communications, Nature, vol. 9(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06908-5
    DOI: 10.1038/s41467-018-06908-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-018-06908-5
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-018-06908-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Qiaoqiao Zhang & Kai Deng & Mengyou Liu & Shengye Yang & Wei Xu & Tong Feng & Minwen Jie & Zhiming Liu & Xiao Sheng & Haiyang Chen & Hao Jiang, 2023. "Phase separation of BuGZ regulates gut regeneration and aging through interaction with m6A regulators," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06908-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.