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A RNA producing DNA hydrogel as a platform for a high performance RNA interference system

Author

Listed:
  • Jaejung Song

    (Pohang University of Science and Technology (POSTECH))

  • Minhyuk Lee

    (Myongji University)

  • Taeyoung Kim

    (Myongji University)

  • Jeongkyeong Na

    (Department of Chemical Engineering, POSTECH)

  • Yebin Jung

    (Department of Chemistry, POSTECH)

  • Gyoo Yeol Jung

    (Pohang University of Science and Technology (POSTECH)
    Department of Chemical Engineering, POSTECH)

  • Sungjee Kim

    (Pohang University of Science and Technology (POSTECH)
    Department of Chemistry, POSTECH)

  • Nokyoung Park

    (Myongji University)

Abstract

RNA interference (RNAi) is a mechanism in which small interfering RNA (siRNA) silences a target gene. Herein, we describe a DNA hydrogel capable of producing siRNA and interfering with protein expression. This RNAi-exhibiting gel (termed I-gel for interfering gel) consists of a plasmid carrying the gene transcribing siRNA against the target mRNA as part of the gel scaffold. The RNAi efficiency of the I-gel has been confirmed by green fluorescent protein (GFP) expression assay and RNA production quantification. The plasmid stability in the I-gel results in an 8-times higher transcription efficiency than that of the free plasmid. We further applied the I-gel to live cells and confirmed its effect in interfering with the GFP expression. The I-gel shows higher RNAi effect than plasmids in free form or complexed with Lipofectamine. This nanoscale hydrogel, which is able to produce RNA in a cell, provides a platform technology for efficient RNAi system.

Suggested Citation

  • Jaejung Song & Minhyuk Lee & Taeyoung Kim & Jeongkyeong Na & Yebin Jung & Gyoo Yeol Jung & Sungjee Kim & Nokyoung Park, 2018. "A RNA producing DNA hydrogel as a platform for a high performance RNA interference system," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06864-0
    DOI: 10.1038/s41467-018-06864-0
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