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Characterization of a long overlooked copper protein from methane- and ammonia-oxidizing bacteria

Author

Listed:
  • Oriana S. Fisher

    (Northwestern University)

  • Grace E. Kenney

    (Northwestern University)

  • Matthew O. Ross

    (Northwestern University)

  • Soo Y. Ro

    (Northwestern University)

  • Betelehem E. Lemma

    (Northwestern University)

  • Sharon Batelu

    (Wayne State University)

  • Paul M. Thomas

    (Northwestern University)

  • Victoria C. Sosnowski

    (Northwestern University)

  • Caroline J. DeHart

    (Northwestern University)

  • Neil L. Kelleher

    (Northwestern University)

  • Timothy L. Stemmler

    (Wayne State University)

  • Brian M. Hoffman

    (Northwestern University)

  • Amy C. Rosenzweig

    (Northwestern University)

Abstract

Methane-oxidizing microbes catalyze the oxidation of the greenhouse gas methane using the copper-dependent enzyme particulate methane monooxygenase (pMMO). Isolated pMMO exhibits lower activity than whole cells, however, suggesting that additional components may be required. A pMMO homolog, ammonia monooxygenase (AMO), converts ammonia to hydroxylamine in ammonia-oxidizing bacteria (AOB) which produce another potent greenhouse gas, nitrous oxide. Here we show that PmoD, a protein encoded within many pmo operons that is homologous to the AmoD proteins encoded within AOB amo operons, forms a copper center that exhibits the features of a well-defined CuA site using a previously unobserved ligand set derived from a cupredoxin homodimer. PmoD is critical for copper-dependent growth on methane, and genetic analyses strongly support a role directly related to pMMO and AMO. These findings identify a copper-binding protein that may represent a missing link in the function of enzymes critical to the global carbon and nitrogen cycles.

Suggested Citation

  • Oriana S. Fisher & Grace E. Kenney & Matthew O. Ross & Soo Y. Ro & Betelehem E. Lemma & Sharon Batelu & Paul M. Thomas & Victoria C. Sosnowski & Caroline J. DeHart & Neil L. Kelleher & Timothy L. Stem, 2018. "Characterization of a long overlooked copper protein from methane- and ammonia-oxidizing bacteria," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
  • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06681-5
    DOI: 10.1038/s41467-018-06681-5
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