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Galanin neurons in the ventrolateral preoptic area promote sleep and heat loss in mice

Author

Listed:
  • Daniel Kroeger

    (Beth Israel Deaconess Medical Center and Harvard Medical School)

  • Gianna Absi

    (Beth Israel Deaconess Medical Center and Harvard Medical School)

  • Celia Gagliardi

    (Beth Israel Deaconess Medical Center and Harvard Medical School)

  • Sathyajit S. Bandaru

    (Beth Israel Deaconess Medical Center and Harvard Medical School)

  • Joseph C. Madara

    (Harvard Medical School)

  • Loris L. Ferrari

    (Beth Israel Deaconess Medical Center and Harvard Medical School)

  • Elda Arrigoni

    (Beth Israel Deaconess Medical Center and Harvard Medical School)

  • Heike Münzberg

    (Louisiana State University System)

  • Thomas E. Scammell

    (Beth Israel Deaconess Medical Center and Harvard Medical School)

  • Clifford B. Saper

    (Beth Israel Deaconess Medical Center and Harvard Medical School)

  • Ramalingam Vetrivelan

    (Beth Israel Deaconess Medical Center and Harvard Medical School)

Abstract

The preoptic area (POA) is necessary for sleep, but the fundamental POA circuits have remained elusive. Previous studies showed that galanin (GAL)- and GABA-producing neurons in the ventrolateral preoptic nucleus (VLPO) express cFos after periods of increased sleep and innervate key wake-promoting regions. Although lesions in this region can produce insomnia, high frequency photostimulation of the POAGAL neurons was shown to paradoxically cause waking, not sleep. Here we report that photostimulation of VLPOGAL neurons in mice promotes sleep with low frequency stimulation (1–4 Hz), but causes conduction block and waking at frequencies above 8 Hz. Further, optogenetic inhibition reduces sleep. Chemogenetic activation of VLPOGAL neurons confirms the increase in sleep, and also reduces body temperature. In addition, chemogenetic activation of VLPOGAL neurons induces short-latency sleep in an animal model of insomnia. Collectively, these findings establish a causal role of VLPOGAL neurons in both sleep induction and heat loss.

Suggested Citation

  • Daniel Kroeger & Gianna Absi & Celia Gagliardi & Sathyajit S. Bandaru & Joseph C. Madara & Loris L. Ferrari & Elda Arrigoni & Heike Münzberg & Thomas E. Scammell & Clifford B. Saper & Ramalingam Vetri, 2018. "Galanin neurons in the ventrolateral preoptic area promote sleep and heat loss in mice," Nature Communications, Nature, vol. 9(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06590-7
    DOI: 10.1038/s41467-018-06590-7
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    Cited by:

    1. Wen Z. Yang & Hengchang Xie & Xiaosa Du & Qian Zhou & Yan Xiao & Zhengdong Zhao & Xiaoning Jia & Jianhui Xu & Wen Zhang & Shuang Cai & Zhangjie Li & Xin Fu & Rong Hua & Junhao Cai & Shuang Chang & Jin, 2023. "A parabrachial-hypothalamic parallel circuit governs cold defense in mice," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    2. Jilin W J L Wang & Fabrizio Lombardi & Xiyun Zhang & Christelle Anaclet & Plamen Ch Ivanov, 2019. "Non-equilibrium critical dynamics of bursts in θ and δ rhythms as fundamental characteristic of sleep and wake micro-architecture," PLOS Computational Biology, Public Library of Science, vol. 15(11), pages 1-35, November.
    3. Sasa Teng & Fenghua Zhen & Li Wang & Jose Canovas Schalchli & Jane Simko & Xinyue Chen & Hao Jin & Christopher D. Makinson & Yueqing Peng, 2022. "Control of non-REM sleep by ventrolateral medulla glutamatergic neurons projecting to the preoptic area," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    4. Roberto Luca & Stefano Nardone & Kevin P. Grace & Anne Venner & Michela Cristofolini & Sathyajit S. Bandaru & Lauren T. Sohn & Dong Kong & Takatoshi Mochizuki & Bianca Viberti & Lin Zhu & Antonino Zit, 2022. "Orexin neurons inhibit sleep to promote arousal," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    5. Shuai Zhang & Xinpei Zhang & Haolin Zhong & Xuanyi Li & Yujie Wu & Jun Ju & Bo Liu & Zhenyu Zhang & Hai Yan & Yizheng Wang & Kun Song & Sheng-Tao Hou, 2022. "Hypothermia evoked by stimulation of medial preoptic nucleus protects the brain in a mouse model of ischaemia," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

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