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Common mechanism of transcription termination at coding and noncoding RNA genes in fission yeast

Author

Listed:
  • Marc Larochelle

    (Université de Sherbrooke)

  • Marc-Antoine Robert

    (Université de Sherbrooke)

  • Jean-Nicolas Hébert

    (Université de Sherbrooke)

  • Xiaochuan Liu

    (Rutgers New Jersey Medical School and Rutgers Cancer Institute of New Jersey)

  • Dominick Matteau

    (Université de Sherbrooke)

  • Sébastien Rodrigue

    (Université de Sherbrooke)

  • Bin Tian

    (Rutgers New Jersey Medical School and Rutgers Cancer Institute of New Jersey)

  • Pierre-Étienne Jacques

    (Université de Sherbrooke
    Université de Sherbrooke)

  • François Bachand

    (Université de Sherbrooke
    Université de Sherbrooke)

Abstract

Termination of RNA polymerase II (RNAPII) transcription is a fundamental step of gene expression that is critical for determining the borders between genes. In budding yeast, termination at protein-coding genes is initiated by the cleavage/polyadenylation machinery, whereas termination of most noncoding RNA (ncRNA) genes occurs via the Nrd1–Nab3–Sen1 (NNS) pathway. Here, we find that NNS-like transcription termination is not conserved in fission yeast. Rather, genome-wide analyses show global recruitment of mRNA 3′ end processing factors at the end of ncRNA genes, including snoRNAs and snRNAs, and that this recruitment coincides with high levels of Ser2 and Tyr1 phosphorylation on the RNAPII C-terminal domain. We also find that termination of mRNA and ncRNA transcription requires the conserved Ysh1/CPSF-73 and Dhp1/XRN2 nucleases, supporting widespread cleavage-dependent transcription termination in fission yeast. Our findings thus reveal that a common mode of transcription termination can produce functionally and structurally distinct types of polyadenylated and non-polyadenylated RNAs.

Suggested Citation

  • Marc Larochelle & Marc-Antoine Robert & Jean-Nicolas Hébert & Xiaochuan Liu & Dominick Matteau & Sébastien Rodrigue & Bin Tian & Pierre-Étienne Jacques & François Bachand, 2018. "Common mechanism of transcription termination at coding and noncoding RNA genes in fission yeast," Nature Communications, Nature, vol. 9(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06546-x
    DOI: 10.1038/s41467-018-06546-x
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    Cited by:

    1. Ebru Aydin & Silke Schreiner & Jacqueline Böhme & Birte Keil & Jan Weber & Bojan Žunar & Timo Glatter & Cornelia Kilchert, 2024. "DEAD-box ATPase Dbp2 is the key enzyme in an mRNP assembly checkpoint at the 3’-end of genes and involved in the recycling of cleavage factors," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    2. Maxime Duval & Carlo Yague-Sanz & Tomasz W. Turowski & Elisabeth Petfalski & David Tollervey & François Bachand, 2023. "The conserved RNA-binding protein Seb1 promotes cotranscriptional ribosomal RNA processing by controlling RNA polymerase I progression," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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