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Paf1 and Ctr9 subcomplex formation is essential for Paf1 complex assembly and functional regulation

Author

Listed:
  • Ying Xie

    (Nankai University)

  • Minying Zheng

    (Nankai University)

  • Xinlei Chu

    (Tianjin Medical University Cancer Institute and Hospital)

  • Yue Chen

    (Nankai University)

  • Huisha Xu

    (Nankai University)

  • Jiawei Wang

    (Tsinghua University)

  • Hao Zhou

    (Nankai University)

  • Jiafu Long

    (Nankai University)

Abstract

The evolutionarily conserved multifunctional polymerase-associated factor 1 (Paf1) complex (Paf1C), which is composed of at least five subunits (Paf1, Leo1, Ctr9, Cdc73, and Rtf1), plays vital roles in gene regulation and has connections to development and human diseases. Here, we report two structures of each of the human and yeast Ctr9/Paf1 subcomplexes, which assemble into heterodimers with very similar conformations, revealing an interface between the tetratricopeptide repeat module in Ctr9 and Paf1. The structure of the Ctr9/Paf1 subcomplex may provide mechanistic explanations for disease-associated mutations in human PAF1 and CTR9. Our study reveals that the formation of the Ctr9/Paf1 heterodimer is required for the assembly of yeast Paf1C, and is essential for yeast viability. In addition, disruption of the interaction between Paf1 and Ctr9 greatly affects the level of histone H3 methylation in vivo. Collectively, our results shed light on Paf1C assembly and functional regulation.

Suggested Citation

  • Ying Xie & Minying Zheng & Xinlei Chu & Yue Chen & Huisha Xu & Jiawei Wang & Hao Zhou & Jiafu Long, 2018. "Paf1 and Ctr9 subcomplex formation is essential for Paf1 complex assembly and functional regulation," Nature Communications, Nature, vol. 9(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06237-7
    DOI: 10.1038/s41467-018-06237-7
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    Cited by:

    1. Negin Khosraviani & V. Talya Yerlici & Jonathan St-Germain & Yi Yang Hou & Shi Bo Cao & Carla Ghali & Michael Bokros & Rehna Krishnan & Razqallah Hakem & Stephen Lee & Brian Raught & Karim Mekhail, 2024. "Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesis," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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