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Ring synthetic chromosome V SCRaMbLE

Author

Listed:
  • Juan Wang

    (Tianjin University
    Tianjin University)

  • Ze-Xiong Xie

    (Tianjin University
    Tianjin University)

  • Yuan Ma

    (Tianjin University
    Tianjin University)

  • Xiang-Rong Chen

    (Tianjin University
    Tianjin University)

  • Yao-Qing Huang

    (Tianjin University
    Tianjin University)

  • Bo He

    (Tianjin University
    Tianjin University)

  • Jia

    (Tianjin University
    Tianjin University)

  • Bing-Zhi Li

    (Tianjin University
    Tianjin University)

  • Ying-Jin Yuan

    (Tianjin University
    Tianjin University)

Abstract

Structural variations (SVs) exert important functional impacts on biological phenotypic diversity. Here we show a ring synthetic yeast chromosome V (ring_synV) can be used to continuously generate complex genomic variations and improve the production of prodeoxyviolacein (PDV) by applying Synthetic Chromosome Recombination and Modification by LoxP-mediated Evolution (SCRaMbLE) in haploid yeast cells. The SCRaMbLE of ring_synV generates aneuploid yeast strains with increased PDV productivity, and we identify aneuploid chromosome I, III, VI, XII, XIII, and ring_synV. The neochromosome of SCRaMbLEd ring_synV generated more unbalanced forms of variations, including duplication, insertions, and balanced forms of translocations and inversions than its linear form. Furthermore, of the 29 novel SVs detected, 11 prompted the PDV biosynthesis; and the deletion of uncharacterized gene YER182W is related to the improvement of the PDV. Overall, the SCRaMbLEing ring_synV embraces the evolution of the genome by modifying the chromosome number, structure, and organization, identifying targets for phenotypic comprehension.

Suggested Citation

  • Juan Wang & Ze-Xiong Xie & Yuan Ma & Xiang-Rong Chen & Yao-Qing Huang & Bo He & Jia & Bing-Zhi Li & Ying-Jin Yuan, 2018. "Ring synthetic chromosome V SCRaMbLE," Nature Communications, Nature, vol. 9(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06216-y
    DOI: 10.1038/s41467-018-06216-y
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    Cited by:

    1. Tiantian Chang & Weichao Ding & Shirui Yan & Yun Wang & Haoling Zhang & Yu Zhang & Zhi Ping & Huiming Zhang & Yijian Huang & Jiahui Zhang & Dan Wang & Wenwei Zhang & Xun Xu & Yue Shen & Xian Fu, 2023. "A robust yeast biocontainment system with two-layered regulation switch dependent on unnatural amino acid," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. Huiming Zhang & Xian Fu & Xuemei Gong & Yun Wang & Haolin Zhang & Yu Zhao & Yue Shen, 2022. "Systematic dissection of key factors governing recombination outcomes by GCE-SCRaMbLE," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    3. Li Cheng & Shijun Zhao & Tianyi Li & Sha Hou & Zhouqing Luo & Jinsheng Xu & Wenfei Yu & Shuangying Jiang & Marco Monti & Daniel Schindler & Weimin Zhang & Chunhui Hou & Yingxin Ma & Yizhi Cai & Jef D., 2024. "Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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