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Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells

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Listed:
  • Angela M. Lager

    (Case Western Reserve University School of Medicine)

  • Olivia G. Corradin

    (Case Western Reserve University School of Medicine)

  • Jared M. Cregg

    (Case Western Reserve University School of Medicine)

  • Matthew S. Elitt

    (Case Western Reserve University School of Medicine)

  • H. Elizabeth Shick

    (Case Western Reserve University School of Medicine)

  • Benjamin L. L. Clayton

    (Case Western Reserve University School of Medicine)

  • Kevin C. Allan

    (Case Western Reserve University School of Medicine)

  • Hannah E. Olsen

    (Case Western Reserve University School of Medicine)

  • Mayur Madhavan

    (Case Western Reserve University School of Medicine)

  • Paul J. Tesar

    (Case Western Reserve University School of Medicine
    Case Western Reserve University School of Medicine)

Abstract

Oligodendrocyte dysfunction underlies many neurological disorders, but rapid assessment of mutation-specific effects in these cells has been impractical. To enable functional genetics in oligodendrocytes, here we report a highly efficient method for generating oligodendrocytes and their progenitors from mouse embryonic and induced pluripotent stem cells, independent of mouse strain or mutational status. We demonstrate that this approach, when combined with genome engineering, provides a powerful platform for the expeditious study of genotype–phenotype relationships in oligodendrocytes.

Suggested Citation

  • Angela M. Lager & Olivia G. Corradin & Jared M. Cregg & Matthew S. Elitt & H. Elizabeth Shick & Benjamin L. L. Clayton & Kevin C. Allan & Hannah E. Olsen & Mayur Madhavan & Paul J. Tesar, 2018. "Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells," Nature Communications, Nature, vol. 9(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06102-7
    DOI: 10.1038/s41467-018-06102-7
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