IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v9y2018i1d10.1038_s41467-018-06073-9.html
   My bibliography  Save this article

A myosin II nanomachine mimicking the striated muscle

Author

Listed:
  • Irene Pertici

    (University of Florence)

  • Lorenzo Bongini

    (University of Florence)

  • Luca Melli

    (University of Florence
    F. Hoffmann-La Roche Ltd)

  • Giulio Bianchi

    (University of Florence)

  • Luca Salvi

    (University of Florence
    University of Munich)

  • Giulia Falorsi

    (University of Florence)

  • Caterina Squarci

    (University of Florence)

  • Tamás Bozó

    (Semmelweis University)

  • Dan Cojoc

    (IOM-CNR)

  • Miklós S. Z. Kellermayer

    (Semmelweis University)

  • Vincenzo Lombardi

    (University of Florence)

  • Pasquale Bianco

    (University of Florence)

Abstract

The contraction of striated muscle (skeletal and cardiac muscle) is generated by ATP-dependent interactions between the molecular motor myosin II and the actin filament. The myosin motors are mechanically coupled along the thick filament in a geometry not achievable by single-molecule experiments. Here we show that a synthetic one-dimensional nanomachine, comprising fewer than ten myosin II dimers purified from rabbit psoas, performs isometric and isotonic contractions at 2 mM ATP, delivering a maximum power of 5 aW. The results are explained with a kinetic model fitted to the performance of mammalian skeletal muscle, showing that the condition for the motor coordination that maximises the efficiency in striated muscle is a minimum of 32 myosin heads sharing a common mechanical ground. The nanomachine offers a powerful tool for investigating muscle contractile-protein physiology, pathology and pharmacology without the potentially disturbing effects of the cytoskeletal—and regulatory—protein environment.

Suggested Citation

  • Irene Pertici & Lorenzo Bongini & Luca Melli & Giulio Bianchi & Luca Salvi & Giulia Falorsi & Caterina Squarci & Tamás Bozó & Dan Cojoc & Miklós S. Z. Kellermayer & Vincenzo Lombardi & Pasquale Bianco, 2018. "A myosin II nanomachine mimicking the striated muscle," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06073-9
    DOI: 10.1038/s41467-018-06073-9
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-018-06073-9
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-018-06073-9?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. C. Arbore & M. Sergides & L. Gardini & G. Bianchi & A. V. Kashchuk & I. Pertici & P. Bianco & F. S. Pavone & M. Capitanio, 2022. "α-catenin switches between a slip and an asymmetric catch bond with F-actin to cooperatively regulate cell junction fluidity," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06073-9. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.