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TBC1d24-ephrinB2 interaction regulates contact inhibition of locomotion in neural crest cell migration

Author

Listed:
  • Jaeho Yoon

    (National Cancer Institute
    Hallym University)

  • Yoo-Seok Hwang

    (National Cancer Institute)

  • Moonsup Lee

    (National Cancer Institute)

  • Jian Sun

    (National Cancer Institute)

  • Hee Jun Cho

    (National Cancer Institute
    Korea Research Institute of Bioscience and Biotechnology (KRIBB))

  • Laura Knapik

    (National Cancer Institute)

  • Ira O. Daar

    (National Cancer Institute)

Abstract

Although Eph-ephrin signalling has been implicated in the migration of cranial neural crest (CNC) cells, it is still unclear how ephrinB transduces signals regulating this event. We provide evidence that TBC1d24, a putative Rab35-GTPase activating protein (Rab35 GAP), complexes with ephrinB2 via the scaffold Dishevelled (Dsh) and mediates a signal affecting contact inhibition of locomotion (CIL) in CNC cells. Moreover, we found that, in migrating CNC, the interaction between ephrinB2 and TBC1d24 negatively regulates E-cadherin recycling in these cells via Rab35. Upon engagement of the cognate Eph receptor, ephrinB2 is tyrosine phosphorylated, which disrupts the ephrinB2/Dsh/TBC1d24 complex. The dissolution of this complex leads to increasing E-cadherin levels at the plasma membrane, resulting in loss of CIL and disrupted CNC migration. Our results indicate that TBC1d24 is a critical player in ephrinB2 control of CNC cell migration via CIL.

Suggested Citation

  • Jaeho Yoon & Yoo-Seok Hwang & Moonsup Lee & Jian Sun & Hee Jun Cho & Laura Knapik & Ira O. Daar, 2018. "TBC1d24-ephrinB2 interaction regulates contact inhibition of locomotion in neural crest cell migration," Nature Communications, Nature, vol. 9(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-05924-9
    DOI: 10.1038/s41467-018-05924-9
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