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CTD-dependent and -independent mechanisms govern co-transcriptional capping of Pol II transcripts

Author

Listed:
  • Melvin Noe Gonzalez

    (Stowers Institute for Medical Research)

  • Shigeo Sato

    (Stowers Institute for Medical Research)

  • Chieri Tomomori-Sato

    (Stowers Institute for Medical Research)

  • Joan W. Conaway

    (Stowers Institute for Medical Research
    Kansas University Medical Center)

  • Ronald C. Conaway

    (Stowers Institute for Medical Research
    Kansas University Medical Center)

Abstract

Co-transcriptional capping of RNA polymerase II (Pol II) transcripts by capping enzyme proceeds orders of magnitude more efficiently than capping of free RNA. Previous studies brought to light a role for the phosphorylated Pol II carboxyl-terminal domain (CTD) in activation of co-transcriptional capping; however, CTD phosphorylation alone could not account for the observed magnitude of activation. Here, we exploit a defined Pol II transcription system that supports both CTD phosphorylation and robust activation of capping to dissect the mechanism of co-transcriptional capping. Taken together, our findings identify a CTD-independent, but Pol II-mediated, mechanism that functions in parallel with CTD-dependent processes to ensure optimal capping, and they support a “tethering” model for the mechanism of activation.

Suggested Citation

  • Melvin Noe Gonzalez & Shigeo Sato & Chieri Tomomori-Sato & Joan W. Conaway & Ronald C. Conaway, 2018. "CTD-dependent and -independent mechanisms govern co-transcriptional capping of Pol II transcripts," Nature Communications, Nature, vol. 9(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-05923-w
    DOI: 10.1038/s41467-018-05923-w
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    Cited by:

    1. Yan Li & Qianmin Wang & Yanhui Xu & Ze Li, 2024. "Structures of co-transcriptional RNA capping enzymes on paused transcription complex," Nature Communications, Nature, vol. 15(1), pages 1-11, December.

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