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De novo targeting to the cytoplasmic and luminal side of bacterial microcompartments

Author

Listed:
  • Matthew J. Lee

    (University of Kent)

  • Judith Mantell

    (University of Bristol, Medical Sciences Building, University Walk
    Medical Sciences Building, University Walk)

  • Ian R. Brown

    (University of Kent)

  • Jordan M. Fletcher

    (University of Bristol, Cantock’s Close)

  • Paul Verkade

    (University of Bristol, Medical Sciences Building, University Walk
    Medical Sciences Building, University Walk
    Life Sciences Building)

  • Richard W. Pickersgill

    (Queen Mary University of London)

  • Derek N. Woolfson

    (University of Bristol, Medical Sciences Building, University Walk
    University of Bristol, Cantock’s Close
    Life Sciences Building)

  • Stefanie Frank

    (University College London)

  • Martin J. Warren

    (University of Kent)

Abstract

Bacterial microcompartments, BMCs, are proteinaceous organelles that encase a specific metabolic pathway within a semi-permeable protein shell. Short encapsulation peptides can direct cargo proteins to the lumen of the compartments. However, the fusion of such peptides to non-native proteins does not guarantee encapsulation and often causes aggregation. Here, we report an approach for targeting recombinant proteins to BMCs that utilizes specific de novo coiled-coil protein–protein interactions. Attachment of one coiled-coil module to PduA (a component of the BMC shell) allows targeting of a fluorescent protein fused to a cognate coiled-coil partner. This interaction takes place on the outer surface of the BMC. The redesign of PduA to generate an N-terminus on the luminal side of the BMC results in intact compartments to which proteins can still be targeted via the designed coiled-coil system. This study provides a strategy to display proteins on the surface or within the lumen of the BMCs.

Suggested Citation

  • Matthew J. Lee & Judith Mantell & Ian R. Brown & Jordan M. Fletcher & Paul Verkade & Richard W. Pickersgill & Derek N. Woolfson & Stefanie Frank & Martin J. Warren, 2018. "De novo targeting to the cytoplasmic and luminal side of bacterial microcompartments," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-05922-x
    DOI: 10.1038/s41467-018-05922-x
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    Cited by:

    1. Carolyn E. Mills & Curt Waltmann & Andre G. Archer & Nolan W. Kennedy & Charlotte H. Abrahamson & Alexander D. Jackson & Eric W. Roth & Sasha Shirman & Michael C. Jewett & Niall M. Mangan & Monica Olv, 2022. "Vertex protein PduN tunes encapsulated pathway performance by dictating bacterial metabolosome morphology," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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