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ESCRT-III mediates budding across the inner nuclear membrane and regulates its integrity

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  • Jun Arii

    (The Institute of Medical Science, The University of Tokyo, Minato-ku
    The Institute of Medical Science, The University of Tokyo, Minato-ku)

  • Mizuki Watanabe

    (The Institute of Medical Science, The University of Tokyo, Minato-ku
    The Institute of Medical Science, The University of Tokyo, Minato-ku)

  • Fumio Maeda

    (The Institute of Medical Science, The University of Tokyo, Minato-ku
    The Institute of Medical Science, The University of Tokyo, Minato-ku)

  • Noriko Tokai-Nishizumi

    (The Institute of Medical Science, The University of Tokyo, Minato-ku)

  • Takahiro Chihara

    (The University of Tokyo
    Hiroshima University, Higashi-Hiroshima)

  • Masayuki Miura

    (The University of Tokyo)

  • Yuhei Maruzuru

    (The Institute of Medical Science, The University of Tokyo, Minato-ku
    The Institute of Medical Science, The University of Tokyo, Minato-ku)

  • Naoto Koyanagi

    (The Institute of Medical Science, The University of Tokyo, Minato-ku
    The Institute of Medical Science, The University of Tokyo, Minato-ku)

  • Akihisa Kato

    (The Institute of Medical Science, The University of Tokyo, Minato-ku
    The Institute of Medical Science, The University of Tokyo, Minato-ku)

  • Yasushi Kawaguchi

    (The Institute of Medical Science, The University of Tokyo, Minato-ku
    The Institute of Medical Science, The University of Tokyo, Minato-ku)

Abstract

Vesicle-mediated nucleocytoplasmic transport is a nuclear pore-independent mechanism for the nuclear export of macromolecular complexes, but the molecular basis for this transport remains largely unknown. Here we show that endosomal sorting complex required for transport-III (ESCRT-III) is recruited to the inner nuclear membrane (INM) during the nuclear export of herpes simplex virus 1 (HSV-1). Scission during HSV-1 budding through the INM is prevented by depletion of ESCRT-III proteins. Interestingly, in uninfected human cells, the depletion of ESCRT-III proteins induces aberrant INM proliferation. Our results show that HSV-1 expropriates the ESCRT-III machinery in infected cells for scission of the INM to produce vesicles containing progeny virus nucleocapsids. In uninfected cells, ESCRT-III regulates INM integrity by downregulating excess INM.

Suggested Citation

  • Jun Arii & Mizuki Watanabe & Fumio Maeda & Noriko Tokai-Nishizumi & Takahiro Chihara & Masayuki Miura & Yuhei Maruzuru & Naoto Koyanagi & Akihisa Kato & Yasushi Kawaguchi, 2018. "ESCRT-III mediates budding across the inner nuclear membrane and regulates its integrity," Nature Communications, Nature, vol. 9(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-05889-9
    DOI: 10.1038/s41467-018-05889-9
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