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Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe

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  • Troy F. Langford

    (Massachusetts Institute of Technology)

  • Beijing K. Huang

    (Massachusetts Institute of Technology)

  • Joseph B. Lim

    (Massachusetts Institute of Technology)

  • Sun Jin Moon

    (Massachusetts Institute of Technology)

  • Hadley D. Sikes

    (Massachusetts Institute of Technology)

Abstract

Redox cancer therapeutics target the increased reliance on intracellular antioxidant systems and enhanced susceptibility to oxidant-induced stress of some cancer cells compared to normal cells. Many of these therapeutics are thought to perturb intracellular levels of the oxidant hydrogen peroxide (H2O2), a signaling molecule that modulates a number of different processes in human cells. However, fluorescent probes for this species remain limited in their ability to detect the small perturbations induced during successful treatments. We report a fluorescent sensor based upon human peroxiredoxin-2, which acts as the natural indicator of small H2O2 fluctuations in human cells. The new probe reveals peroxide-induced oxidation in human cells below the detection limit of current probes, as well as peroxiredoxin-2 oxidation caused by two different redox cancer therapeutics in living cells. This capability will be useful in elucidating the mechanism of current redox-based therapeutics and in developing new ones.

Suggested Citation

  • Troy F. Langford & Beijing K. Huang & Joseph B. Lim & Sun Jin Moon & Hadley D. Sikes, 2018. "Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-05557-y
    DOI: 10.1038/s41467-018-05557-y
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