IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v9y2018i1d10.1038_s41467-018-04827-z.html
   My bibliography  Save this article

Identification of dynamic undifferentiated cell states within the male germline

Author

Listed:
  • Hue M. La

    (Australian Regenerative Medicine Institute, Monash University
    Monash University)

  • Juho-Antti Mäkelä

    (Australian Regenerative Medicine Institute, Monash University
    Monash University
    Research Centre for Integrative Physiology and Pharmacology, University of Turku)

  • Ai-Leen Chan

    (Australian Regenerative Medicine Institute, Monash University
    Monash University)

  • Fernando J. Rossello

    (Australian Regenerative Medicine Institute, Monash University
    Monash University)

  • Christian M. Nefzger

    (Australian Regenerative Medicine Institute, Monash University
    Monash University)

  • Julien M. D. Legrand

    (Australian Regenerative Medicine Institute, Monash University
    Monash University)

  • Mia Seram

    (Australian Regenerative Medicine Institute, Monash University
    Monash University)

  • Jose M. Polo

    (Australian Regenerative Medicine Institute, Monash University
    Monash University)

  • Robin M. Hobbs

    (Australian Regenerative Medicine Institute, Monash University
    Monash University)

Abstract

The role of stem cells in tissue maintenance is appreciated and hierarchical models of stem cell self-renewal and differentiation often proposed. Stem cell activity in the male germline is restricted to undifferentiated A-type spermatogonia (Aundiff); however, only a fraction of this population act as stem cells in undisturbed testis and Aundiff hierarchy remains contentious. Through newly developed compound reporter mice, here we define molecular signatures of self-renewing and differentiation-primed adult Aundiff fractions and dissect Aundiff heterogeneity by single-cell analysis. We uncover an unappreciated population within the self-renewing Aundiff fraction marked by expression of embryonic patterning genes and homeodomain transcription factor PDX1. Importantly, we find that PDX1 marks a population with potent stem cell capacity unique to mature, homeostatic testis and demonstrate dynamic interconversion between PDX1+ and PDX1− Aundiff states upon transplant and culture. We conclude that Aundiff exist in a series of dynamic cell states with distinct function and provide evidence that stability of such states is dictated by niche-derived cues.

Suggested Citation

  • Hue M. La & Juho-Antti Mäkelä & Ai-Leen Chan & Fernando J. Rossello & Christian M. Nefzger & Julien M. D. Legrand & Mia Seram & Jose M. Polo & Robin M. Hobbs, 2018. "Identification of dynamic undifferentiated cell states within the male germline," Nature Communications, Nature, vol. 9(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04827-z
    DOI: 10.1038/s41467-018-04827-z
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-018-04827-z
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-018-04827-z?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Hue M. La & Jinyue Liao & Julien M. D. Legrand & Fernando J. Rossello & Ai-Leen Chan & Vijesh Vaghjiani & Jason E. Cain & Antonella Papa & Tin Lap Lee & Robin M. Hobbs, 2022. "Distinctive molecular features of regenerative stem cells in the damaged male germline," Nature Communications, Nature, vol. 13(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04827-z. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.