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ZOLA-3D allows flexible 3D localization microscopy over an adjustable axial range

Author

Listed:
  • Andrey Aristov

    (Institut Pasteur
    UMR 3691, CNRS; C3BI, USR 3756, IP CNRS)

  • Benoit Lelandais

    (Institut Pasteur
    UMR 3691, CNRS; C3BI, USR 3756, IP CNRS
    Institut Pasteur)

  • Elena Rensen

    (Institut Pasteur
    UMR 3691, CNRS; C3BI, USR 3756, IP CNRS)

  • Christophe Zimmer

    (Institut Pasteur
    UMR 3691, CNRS; C3BI, USR 3756, IP CNRS)

Abstract

Single molecule localization microscopy can generate 3D super-resolution images without scanning by leveraging the axial variations of normal or engineered point spread functions (PSF). Successful implementation of these approaches for extended axial ranges remains, however, challenging. We present Zernike Optimized Localization Approach in 3D (ZOLA-3D), an easy-to-use computational and optical solution that achieves optimal resolution over a tunable axial range. We use ZOLA-3D to demonstrate 3D super-resolution imaging of mitochondria, nuclear pores and microtubules in entire nuclei or cells up to ~5 μm deep.

Suggested Citation

  • Andrey Aristov & Benoit Lelandais & Elena Rensen & Christophe Zimmer, 2018. "ZOLA-3D allows flexible 3D localization microscopy over an adjustable axial range," Nature Communications, Nature, vol. 9(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04709-4
    DOI: 10.1038/s41467-018-04709-4
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