IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v9y2018i1d10.1038_s41467-018-04677-9.html
   My bibliography  Save this article

Cotransporter-mediated water transport underlying cerebrospinal fluid formation

Author

Listed:
  • Annette B. Steffensen

    (University of Copenhagen)

  • Eva K. Oernbo

    (University of Copenhagen)

  • Anca Stoica

    (University of Copenhagen)

  • Niklas J. Gerkau

    (Heinrich Heine University Duesseldorf)

  • Dagne Barbuskaite

    (University of Copenhagen)

  • Katerina Tritsaris

    (University of Copenhagen)

  • Christine R. Rose

    (Heinrich Heine University Duesseldorf)

  • Nanna MacAulay

    (University of Copenhagen)

Abstract

Cerebrospinal fluid (CSF) production occurs at a rate of 500 ml per day in the adult human. Conventional osmotic forces do not suffice to support such production rate and the molecular mechanisms underlying this fluid production remain elusive. Using ex vivo choroid plexus live imaging and isotope flux in combination with in vivo CSF production determination in mice, we identify a key component in the CSF production machinery. The Na+/K+/2Cl− cotransporter (NKCC1) expressed in the luminal membrane of choroid plexus contributes approximately half of the CSF production, via its unusual outward transport direction and its unique ability to directly couple water transport to ion translocation. We thereby establish the concept of cotransport of water as a missing link in the search for molecular pathways sustaining CSF production and redefine the current model of this pivotal physiological process. Our results provide a rational pharmacological target for pathologies involving disturbed brain fluid dynamics.

Suggested Citation

  • Annette B. Steffensen & Eva K. Oernbo & Anca Stoica & Niklas J. Gerkau & Dagne Barbuskaite & Katerina Tritsaris & Christine R. Rose & Nanna MacAulay, 2018. "Cotransporter-mediated water transport underlying cerebrospinal fluid formation," Nature Communications, Nature, vol. 9(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04677-9
    DOI: 10.1038/s41467-018-04677-9
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-018-04677-9
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-018-04677-9?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04677-9. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.