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CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing

Author

Listed:
  • Toon Swings

    (KU Leuven - University of Leuven
    Center for Microbiology)

  • David C. Marciano

    (Baylor College of Medicine)

  • Benu Atri

    (Baylor College of Medicine)

  • Rachel E. Bosserman

    (McGovern Medical School)

  • Chen Wang

    (Baylor College of Medicine)

  • Marlies Leysen

    (KU Leuven - University of Leuven)

  • Camille Bonte

    (KU Leuven - University of Leuven)

  • Thomas Schalck

    (KU Leuven - University of Leuven
    Center for Microbiology)

  • Ian Furey

    (Baylor College of Medicine)

  • Bram Van den Bergh

    (KU Leuven - University of Leuven
    Center for Microbiology)

  • Natalie Verstraeten

    (KU Leuven - University of Leuven
    Center for Microbiology)

  • Peter J. Christie

    (McGovern Medical School)

  • Christophe Herman

    (Baylor College of Medicine)

  • Olivier Lichtarge

    (Baylor College of Medicine
    Baylor College of Medicine
    Baylor College of Medicine
    Baylor College of Medicine)

  • Jan Michiels

    (KU Leuven - University of Leuven
    Center for Microbiology)

Abstract

CRISPR advances genome engineering by directing endonuclease sequence specificity with a guide RNA molecule (gRNA). For precisely targeting a gene for modification, each genetic construct requires a unique gRNA. By generating a gRNA against the flippase recognition target (FRT) site, a common genetic element shared by multiple genetic collections, CRISPR-FRT circumvents this design constraint to provide a broad platform for fast, scarless, off-the-shelf genome engineering.

Suggested Citation

  • Toon Swings & David C. Marciano & Benu Atri & Rachel E. Bosserman & Chen Wang & Marlies Leysen & Camille Bonte & Thomas Schalck & Ian Furey & Bram Van den Bergh & Natalie Verstraeten & Peter J. Christ, 2018. "CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04651-5
    DOI: 10.1038/s41467-018-04651-5
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    Cited by:

    1. Liselot Dewachter & Aaron N. Brooks & Katherine Noon & Charlotte Cialek & Alia Clark-ElSayed & Thomas Schalck & Nandini Krishnamurthy & Wim Versées & Wim Vranken & Jan Michiels, 2023. "Deep mutational scanning of essential bacterial proteins can guide antibiotic development," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    2. Liselot Dewachter & Babette Deckers & Israel Mares-Mejía & Elen Louwagie & Silke Vercauteren & Paul Matthay & Simon Brückner & Anna-Maria Möller & Franz Narberhaus & Sibylle C. Vonesch & Wim Versées &, 2024. "The role of the essential GTPase ObgE in regulating lipopolysaccharide synthesis in Escherichia coli," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    3. David C. Marciano & Chen Wang & Teng-Kuei Hsu & Thomas Bourquard & Benu Atri & Ralf B. Nehring & Nicholas S. Abel & Elizabeth A. Bowling & Taylor J. Chen & Pamela D. Lurie & Panagiotis Katsonis & Susa, 2022. "Evolutionary action of mutations reveals antimicrobial resistance genes in Escherichia coli," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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